Peng L-Y, Wang J, Tao M, You C-P, Ye L, Xiao J, Zhang C, Liu Y, Liu S-J
Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Curr Mol Med. 2014;14(10):1314-21. doi: 10.2174/1566524014666141203095503.
An allotetraploid hybrid lineage derived from the distant hybridization of red crucian carp (Carassius auratus red var., ♀, 2n =100) × common carp (Cyprinus carpio L., ♂, 2n =100) was investigated for its mitochondrial and nuclear genome inheritance patterns. Based on liver transcriptomic data for this hybrid, red crucian carp, and common carp, we identified 94, 136, and 86 contigs corresponding to 41, 46, and 37 mitochondrial respiratory chain nuclear genes, respectively. Mitochondrial respiratory chain nuclear gene sequences from red crucian carp and common carp were both detected in the allotetraploid hybrid, indicating that both parental nuclear genomes were participated in the synthesis of mitochondrial respiratory protein complexes in the hybrid. For mitochondrial respiratory related genes, high sequence similarity (>90%) and a low nucleotide divergence rate (<0.2) between red crucian carp and common carp could be a critical factor allowing cooperation of the three genomes (red crucian carp mitochondrial genome, red crucian and common carp nuclear genomes) in the allotetraploid hybrid lineage. Interestingly, gene duplication events were identified in the allotetraploid hybrid, red crucian and common carp, as confirmed by analysis of orthologous gene trees for these fish. Our findings provide valuable information with which to study cooperation between the nuclear and mitochondrial genomes of other hybrids, and will provide basic genetic information of relevance to mitochondrial-related diseases in humans and animals.
对由红鲫（Carassius auratus red var.，♀，2n = 100）与鲤鱼（Cyprinus carpio L.，♂，2n = 100）远缘杂交产生的异源四倍体杂交谱系的线粒体和核基因组遗传模式进行了研究。基于该杂交种、红鲫和鲤鱼的肝脏转录组数据，我们分别鉴定出了94个、136个和86个重叠群，它们分别对应41个、46个和37个线粒体呼吸链核基因。在异源四倍体杂交种中检测到了来自红鲫和鲤鱼的线粒体呼吸链核基因序列，这表明两个亲本的核基因组都参与了杂交种中线粒体呼吸蛋白复合物的合成。对于线粒体呼吸相关基因，红鲫和鲤鱼之间较高的序列相似性（>90%）和较低的核苷酸分歧率（<0.2）可能是异源四倍体杂交谱系中三个基因组（红鲫线粒体基因组、红鲫和鲤鱼核基因组）能够协同作用的关键因素。有趣的是，通过对这些鱼类直系同源基因树的分析证实，在异源四倍体杂交种、红鲫和鲤鱼中都发现了基因复制事件。我们的研究结果为研究其他杂交种的核基因组与线粒体基因组之间的协同作用提供了有价值的信息，并将为人类和动物线粒体相关疾病提供相关的基础遗传信息。
