Condorelli D F, Kaczmarek L, Nicoletti F, Arcidiacono A, Dell'Albani P, Ingrao F, Magrì G, Malaguarnera L, Avola R, Messina A
Institute of Biochemistry, Faculty of Medicine, University of Catania, Italy.
J Neurosci Res. 1989 Jun;23(2):234-9. doi: 10.1002/jnr.490230214.
In the present study various extracellular factors, acting through different second messenger systems, were examined for their capacity to increase the level of c-fos mRNA in primary glial cell cultures. In particular EGF, 12-O-tetradecanoylphorbol 13-acetate, the beta-adrenergic agonist isoproterenol, and the glutamate agonists, ibotenic and quisqualic acid, were studied. All the extracellular stimuli tested induced a rapid and transient increase in c-fos mRNA level in glial cell cultures regardless of the signal transduction pathway and the final effect on cell proliferation.
在本研究中，对各种通过不同第二信使系统起作用的细胞外因子进行了检测，以考察它们提高原代神经胶质细胞培养物中c-fos mRNA水平的能力。特别研究了表皮生长因子（EGF）、12-O-十四烷酰佛波醇-13-乙酸酯、β-肾上腺素能激动剂异丙肾上腺素以及谷氨酸激动剂鹅膏蕈氨酸和喹啉酸。所有测试的细胞外刺激物均能诱导神经胶质细胞培养物中c-fos mRNA水平迅速且短暂地升高，而与信号转导途径以及对细胞增殖的最终影响无关。
