Arenander A T, Lim R W, Varnum B C, Cole R, de Vellis J, Herschman H R
Department of Biological Chemistry, UCLA School of Medicine, University of California.
J Neurosci Res. 1989 Jul;23(3):247-56. doi: 10.1002/jnr.490230302.
The expression of a number of TIS genes (Lim et al.: Oncogene 1:263-270, 1987) was examined in secondary cultures of rat neocortical astrocytes treated with mitogens and stellation agents, to study the early nuclear events which accompany the induction of glial proliferation and/or differentiation. Tetradecanoyl phorbol acetate (TPA), epidermal growth factor, and fibroblast growth factor, three mitogens for astrocytes, stimulated marked, rapid, and transient increases in TIS gene mRNAS. TIS10, which is not expressed in rat PC12 pheochromocytoma cells, could be induced by these mitogens in rat astrocytes. Dibutyryl cyclic adenosine monophosphate and forskolin, which induce rapid stellation in astrocytes, and ganglioside GM1, a potent mitogen as well as an antagonist of the induction and maintenance of stellation, all induced TIS gene expression. Thus, a broad range of agents which elicit both proliferative and differentiation responses in astrocytes are capable of inducing a family of genes that may play a role in the early events of signal transduction.
在经有丝分裂原和星形化剂处理的大鼠新皮质星形胶质细胞的传代培养物中，检测了一些TIS基因（Lim等人：《癌基因》1:263 - 270，1987年）的表达，以研究伴随胶质细胞增殖和/或分化诱导的早期核事件。十四酰佛波醇乙酸酯（TPA）、表皮生长因子和成纤维细胞生长因子，这三种星形胶质细胞的有丝分裂原，刺激了TIS基因mRNA的显著、快速和短暂增加。在大鼠嗜铬细胞瘤PC12细胞中不表达的TIS10，可被这些有丝分裂原在大鼠星形胶质细胞中诱导表达。二丁酰环磷酸腺苷和福斯高林，它们可诱导星形胶质细胞快速星形化，以及神经节苷脂GM1，一种有效的有丝分裂原以及星形化诱导和维持的拮抗剂，均诱导了TIS基因表达。因此，一系列能在星形胶质细胞中引发增殖和分化反应的物质，都能够诱导一个可能在信号转导早期事件中起作用的基因家族。
