Zhang Hui-ye, Xu Xiao-fei, Huang Yong, Wamg De-qin, Deng Qiao-hua
Zhong Yao Cai. 2014 Jun;37(6):1055-8.
To establish an analytical method for characteristic fingerprint and determination of main components of Andrographis paniculata Extract by UPLC.
The chromatographic conditions were Waters ACQUITY UPLC BEH-C18 (2. 1 mm x 0 mm,1.7 μm)by gradient elute using acetonitrile-water as mobile phase(0 -2 min,20% ~ 25% A;2 ~ 5 min,25% ~ 35% A;5 ~ 7 min,35% A;7 10 min,35% 55% A) at a flow rate of 0. 5 min/mL,detecting wavelength at 220 nm. Results:Contents of the andrographolide, neoandrographolide, 14-deoxyandrographolide and 14-deoxy-l11,12-didehydroandrographolide had good resolution with the correlation coefficients exceed 0. 9999 and the average percent recovery lied in 97. 2% to 103.9%, RSD was less than 3.0% (n = 6). The chromatograms of Andrographis paniculata Extract shared seven common peaks in which four of them were recognized by reference standard with the similarities over 0. 9.
It is a fast,accurate and validated method,and can be useful in quality evaluations of Andrographis paniculata Extract.
建立穿心莲提取物特征指纹图谱及主要成分含量测定的超高效液相色谱分析方法。
采用Waters ACQUITY UPLC BEH-C18色谱柱（2.1 mm×0 mm，1.7μm），以乙腈-水为流动相进行梯度洗脱（0～2分钟，20%～25% A；2～5分钟，25%～35% A；5～7分钟，35% A；7～10分钟，35%～55% A），流速为0.5毫升/分钟，检测波长为220纳米。结果：穿心莲内酯、新穿心莲内酯、14-去氧穿心莲内酯和14-去氧-11,12-二脱氢穿心莲内酯的含量具有良好的分离度，相关系数均大于0.9999，平均回收率在97.2%～103.9%之间，相对标准偏差小于3.0%（n = 6）。穿心莲提取物的色谱图共有7个共有峰，其中4个经对照品确认，相似度均大于0.9。
该方法快速、准确、可靠，可用于穿心莲提取物的质量评价。
