Oaknin S, Rodriguez del Castillo A, Guerra M, Battaner E, Mas M
Department of Biochemistry, University of La Laguna, Tenerife, Spain.
Physiol Behav. 1989 Feb;45(2):407-10. doi: 10.1016/0031-9384(89)90148-0.
We have previously shown that Na,K-ATPase activity (the enzymatic machinery for the sodium pump) in brain areas such as the medial basal hypothalamus (MBH) and the preoptic-suprachiasmatic region (POSC) can be changed by experimental manipulations of gonadal function. We now report enzyme levels in brain regions as related to hormonal changes occurring during sexual behavior. Male rats were exposed to receptive females and decapitated immediately after displaying one of the following behavioral events: the start of copulatory activity, first ejaculation, and the beginning of a second copulatory series. A group of noncopulating animals were used as control. The variables measured included serum levels of LH, PRL and testosterone and Na,K-ATPase activity in MBH, POSC and parietal cerebral cortex (CC). A steady increase in enzyme activity in the POSC, but not the MBH or CC, was found in copulating animals. Serum LH levels changed in a similar fashion. A sharp increase in serum PRL levels, seemingly related to ejaculation, was also observed. These data are consistent with our previous findings on monoaminergic neurotransmission in brain regions related to male sexual behavior.
我们之前已经表明，性腺功能的实验性操作可以改变内侧基底下丘脑（MBH）和视前-视交叉上区（POSC）等脑区的钠钾-ATP酶活性（钠泵的酶机制）。我们现在报告与性行为期间发生的激素变化相关的脑区酶水平。将雄性大鼠暴露于处于接受状态的雌性大鼠中，并在出现以下行为事件之一后立即断头：交配活动开始、首次射精以及第二次交配系列开始。一组未交配的动物用作对照。所测量的变量包括血清促黄体生成素（LH）、催乳素（PRL）和睾酮水平，以及MBH、POSC和顶叶大脑皮层（CC）中的钠钾-ATP酶活性。在交配的动物中，发现POSC中的酶活性稳步增加，但MBH或CC中没有。血清LH水平以类似方式变化。还观察到血清PRL水平急剧升高，这似乎与射精有关。这些数据与我们之前关于与雄性性行为相关的脑区单胺能神经传递的研究结果一致。
