Dvorák M
Vet Med (Praha). 1989 May;34(5):307-16.
The adsorption of aflatoxin (AF) B1, contained in aqueous medium or in synthetic medium left after the cultivation of Aspergillus parasiticus NRRL 2999, was studied in two samples of bentonite, two samples of natural zeolite, and three kinds of adsorption coal added to water, to saline, to the blood serum of pigs, to the stomach fluid of pigs or rumen fluid of cows at concentrations of 5 to 50 mg per litre. Unlike retinol-propionate and beta-carotene, AF was readily adsorbable in vitro. The initial concentration of 3.6 mg or 18 mg AFB1 per litre was reduced to 0.3-27% after exposure to 50 g of adsorbent; this reduction was greater when the AFB1 was tested in the cultivation medium and the maximum reduction was recorded when adsorption coals was involved. The effectiveness of zeolite was lower in media containing nitrogen compounds and in those cases when its doses were low. The average AF retention in the adsorbents, calculated after the determination of desorption by chloroform extraction, was 72% of the original content in the medium in the case of adsorption coals, 66% in bentonite, and 60% in zeolite.
研究了在每升浓度为5至50毫克的情况下，将两种膨润土样品、两种天然沸石样品以及三种吸附煤添加到水、盐水、猪血清、猪胃液或牛瘤胃液中后，对寄生曲霉NRRL 2999培养后剩余的水相介质或合成介质中所含黄曲霉毒素（AF）B1的吸附情况。与视黄醇丙酸酯和β-胡萝卜素不同，AF在体外易于吸附。每升3.6毫克或18毫克AFB1的初始浓度在接触50克吸附剂后降至0.3 - 27%；当在培养基中测试AFB1时，这种降低幅度更大，而涉及吸附煤时记录到的降低幅度最大。在含有氮化合物的介质中以及沸石剂量较低的情况下，沸石的有效性较低。通过氯仿萃取测定解吸后计算得出，吸附煤对AF的平均保留率为介质中原始含量的72%，膨润土为66%，沸石为60%。
