简明综述：人成体干细胞体外向肝样细胞的分化。
Concise Review: Differentiation of Human Adult Stem Cells Into Hepatocyte-like Cells In vitro.
机构信息
Department of Gastroenterology, Institute of Geriatrics, Chinese PLA General Hospital, Beijing, China.
Transfusion Blood Department, Chinese PLA General Hospital, Beijing, China.
出版信息
Int J Stem Cells. 2014 Nov;7(2):49-54. doi: 10.15283/ijsc.2014.7.2.49.
Adult stem cells (ASCs) are undifferentiated cells found throughout the body that divide to replenish dying cells and regenerate damaged tissues, which are the powerful sources for cell therapy and tissue engineering. Bone marrow-derived mesenchymal stem cells (BMSCs), adipose tissue-derived mesenchymal stem cells (ADSCs), and peripheral blood monocytes (PBMCs) are the common ASCs, and many studies indicated that ASCs isolated from various adult tissues could be induced to hepatocyte-like cells in vitro. However, the isolation, culture protocols, characterization of ASCs and hepatocyte-like cells are different. This review aims to describe the isolation and culture procedures for ASCs, to summarize the molecular characterization of ASCs, to characterize function of hepatocyte-like cells, and to discuss the future role of ASCs in cell therapy and tissue engineering.
成体干细胞（ASCs）是存在于全身的未分化细胞，可分裂以补充死亡细胞并再生受损组织，是细胞治疗和组织工程的强大来源。骨髓间充质干细胞（BMSCs）、脂肪组织来源的间充质干细胞（ADSCs）和外周血单核细胞（PBMCs）是常见的 ASCs，许多研究表明，从各种成人组织中分离的 ASCs 可在体外诱导为肝样细胞。然而，ASCs 和肝样细胞的分离、培养方案和特征鉴定是不同的。本综述旨在描述 ASCs 的分离和培养程序，总结 ASCs 的分子特征，描述肝样细胞的功能，并讨论 ASCs 在细胞治疗和组织工程中的未来作用。
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