热带假丝酵母细胞在乙醇诱导丝状生长早期cAMP的积累及其被肌醇抑制的情况。

Accumulation of cAMP in the cells of Candida tropicalis at an early stage of ethanol-induced filamentous growth and its prevention by myo-inositol.

作者信息

Omi K, Kamihara T

机构信息

Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Japan.

出版信息

Biochem Biophys Res Commun. 1989 Jul 31;162(2):646-50. doi: 10.1016/0006-291x(89)92359-0.

DOI:10.1016/0006-291x(89)92359-0

PMID:2547368

Abstract

Phosphatidylinositol metabolism is enhanced in the cells of Candida tropicalis Pk 233 at an early stage of filamentous growth caused by ethanol, and myo-inositol prevents the ethanol-induced changes in the metabolism and morphology [Uejima et al. (1987) FEBS Lett. 214, 127-129]. The accumulation of cAMP and an increase in adenylate cyclase activity were observed in the cells grown with ethanol to the mid-log phase. Myo-inositol abolished these effects of ethanol also. The activity of cAMP phosphodiesterase was affected by neither ethanol nor myo-inositol. These results suggest that the inositol phospholipid-linked and cAMP-linked signaling pathways may be involved in the mechanism of ethanol-induced filamentous growth of this yeast and also that myo-inositol would affect morphogenesis by controlling these pathways.

摘要

在热带假丝酵母Pk 233细胞中，乙醇诱导丝状生长的早期阶段磷脂酰肌醇代谢增强，而肌醇可阻止乙醇诱导的代谢和形态变化[上岛等人（1987年）《欧洲生物化学学会联合会快报》214, 127 - 129]。在乙醇培养至对数中期的细胞中观察到cAMP的积累和腺苷酸环化酶活性的增加。肌醇也消除了乙醇的这些作用。cAMP磷酸二酯酶的活性既不受乙醇影响，也不受肌醇影响。这些结果表明，肌醇磷脂连接和cAMP连接的信号通路可能参与了乙醇诱导该酵母丝状生长的机制，并且肌醇可能通过控制这些通路影响形态发生。