通过pUC载体在大肠杆菌中表达的蓝舌病毒17型融合蛋白ns1。
Bluetongue virus-17 fusion protein ns1 expressed in Escherichia coli by pUC vectors.
作者信息
Wang L F, Doi R H, Chuang L F, Osburn B I, Maisonnave J, Benjamini E, Chuang R Y
机构信息
Department of Biochemistry & Biophysics, University of California, Davis 95616.
出版信息
Biochem Biophys Res Commun. 1989 Jul 31;162(2):892-9. doi: 10.1016/0006-291x(89)92393-0.
The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a LacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.
此前已克隆出编码蓝舌病血清型17（BTV - 17）主要非结构蛋白NS1的互补DNA（cDNA）。利用pUC质粒，我们已成功在大肠杆菌中表达出NS1蛋白，其形式为LacZ - NS1融合蛋白。重组NS1蛋白与兔抗BTV - 17抗血清发生反应，因此在免疫学上与天然BTV - 17 NS1蛋白无法区分。这是首个在细菌系统中产生的蓝舌病病毒蛋白。
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