蓝舌病病毒17型全长非结构蛋白NS1在大肠杆菌中的表达

Expression of a full-length nonstructural protein NS1 of bluetongue virus serotype 17 in Escherichia coli.

作者信息

He X S, Wang L F, Doi R H, Maia M, Osburn B I, Chuang R Y

机构信息

Department of Biochemistry and Biophysics, University of California, Davis 95616.

出版信息

Biochem Biophys Res Commun. 1991 Oct 31;180(2):994-1001. doi: 10.1016/s0006-291x(05)81164-7.

DOI:10.1016/s0006-291x(05)81164-7

PMID:1659409

Abstract

The relative abundance of the nonstructural protein NS1 in bluetongue virus (BTV)-infected cells, the existence of NS1 in the BTV particles and the highly conserved NS1 gene among BTV serotypes indicate the diagnostic potential of using NS1 in detecting BTV infections. In this study a NS1 gene was expressed with the T7 RNA polymerase expression system to produce a full-length NS1 protein. Sheep anti-NS1 antibodies were raised with the E. coli-produced NS1 and used to show that the NS1 proteins of the five BTV serotypes in the Unites States were immunologically indistinguishable.

摘要

蓝舌病病毒（BTV）感染细胞中非结构蛋白NS1的相对丰度、NS1在BTV病毒颗粒中的存在以及BTV血清型间高度保守的NS1基因，表明了利用NS1检测BTV感染的诊断潜力。在本研究中，用T7 RNA聚合酶表达系统表达NS1基因以产生全长NS1蛋白。用大肠杆菌产生的NS1制备绵羊抗NS1抗体，并用于证明美国五种BTV血清型的NS1蛋白在免疫上无法区分。