Tanizawa H, Tai H H
Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Kentucky, Lexington 40536-0082.
Biochem Pharmacol. 1989 Aug 1;38(15):2559-63. doi: 10.1016/0006-2952(89)90102-0.
Formyl-Met-Leu-Phe (FMLP) and platelet-activating factor (PAF) were capable of stimulating thromboxane B2 (TXB2) and leukotriene B4 (LTB4) syntheses in human neutrophils, albeit in a relatively poor degree. A combination of FMLP and PAF, however, was synergistic in stimulating TXB2 and LTB4 syntheses. Phorbol myristate acetate (PMA) appeared to attenuate PAF- but not FMLP-induced arachidonate metabolism. These results suggest that cooperative action of FMLP and PAF on arachidonate release and metabolism does exist and that PMA-mediated protein kinase C activation may regulate FMLP and PAF actions in a different manner.
甲酰甲硫氨酸亮氨酸苯丙氨酸（FMLP）和血小板活化因子（PAF）能够刺激人中性粒细胞合成血栓素B2（TXB2）和白三烯B4（LTB4），尽管程度相对较低。然而，FMLP和PAF的组合在刺激TXB2和LTB4合成方面具有协同作用。佛波酯（PMA）似乎可减弱PAF诱导的花生四烯酸代谢，但对FMLP诱导的花生四烯酸代谢无影响。这些结果表明，FMLP和PAF在花生四烯酸释放和代谢上确实存在协同作用，并且PMA介导的蛋白激酶C激活可能以不同方式调节FMLP和PAF的作用。
