采用直接活菌计数荧光原位杂交技术（DVC-FISH）对地中海一条大河中的幽门螺杆菌进行分子检测。

Molecular detection of Helicobacter pylori in a large Mediterranean river, by direct viable count fluorescent in situ hybridization (DVC-FISH).

作者信息

Tirodimos Ilias, Bobos Mattheos, Kazakos Evangelos, Haidich Anna-Bettina, Dardavessis Theodore, Kostopoulos Ioannis, Arvanitidou Malamatenia

机构信息

Laboratory of Hygiene and Epidemiology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece E-mail:

Department of Pathology, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece.

出版信息

J Water Health. 2014 Dec;12(4):868-73. doi: 10.2166/wh.2014.171.

DOI:10.2166/wh.2014.171

PMID:25473996

Abstract

Although the precise route and mode of transmission of Helicobacter pylori are still unclear, molecular methods have been applied for the detection of H. pylori in environmental samples. In this study, we used the direct viable count fluorescent in situ hybridization (DVC-FISH) method to detect viable cells of H. pylori in the River Aliakmon, Greece. This is the longest river in Greece, and provides potable water in metropolitan areas. H. pylori showed positive detection for 23 out of 48 water samples (47.9%), while no seasonal variation was found and no correlation was observed between the presence of H. pylori and indicators of fecal contamination. Our findings strengthen the evidence that H. pylori is waterborne while its presence adds to the potential health hazards of the River Aliakmon.

摘要

尽管幽门螺杆菌的确切传播途径和方式仍不明确，但分子方法已被应用于环境样本中幽门螺杆菌的检测。在本研究中，我们使用直接活菌计数荧光原位杂交（DVC-FISH）方法检测希腊阿利亚克蒙河水中幽门螺杆菌的活菌。这是希腊最长的河流，为大都市地区提供饮用水。在48份水样中，有23份（47.9%）幽门螺杆菌检测呈阳性，未发现季节性变化，且未观察到幽门螺杆菌的存在与粪便污染指标之间存在相关性。我们的研究结果进一步证明了幽门螺杆菌是通过水传播的，其存在增加了阿利亚克蒙河潜在的健康危害。