Porres-Martínez M, González-Burgos E, Carretero M E, Gómez-Serranillos M P
Department of Pharmacology, Faculty of Pharmacy, University Complutense of Madrid , Spain.
Pharm Biol. 2015 Jun;53(6):921-9. doi: 10.3109/13880209.2014.950672. Epub 2014 Dec 4.
Salvia lavandulifolia has been employed in folk medicine for the treatment of memory and dementia problems. This specie contains numerous bioactive terpenes which may contribute to its effectiveness.
To analyze the composition of essential oil of S. lavandulifolia and to investigate the potential in vitro cytoprotective and antioxidant activities of its major compounds, α-pinene and 1,8-cineole, against H2O2-induced oxidative stress in the U373-MG cell line.
Chemical composition was analyzed by gas chromatography; antioxidant capacity was measured using the ORAC assay, and cytoprotective activity was evaluated using the MTT assay (for cell viability) (range of concentrations: 10-400 μM), DCFH-DA assay (for intracellular ROS generation), thiobarbituric acid reactive substances (TBARS) method (for lipid peroxidation), and spectrofometric techniques and Western blot (for enzymatic activity and protein expression, respectively) at 10 and 25 µM.
α-Pinene (18.39%) and 1,8-cineole (19.57%) were identified as major compounds in S. lavandulifolia essential oil. Pretreatments with these monoterpenes protected U373-MG cells against H2O2-induced oxidative injury by attenuating the loss of cell viability (IC50 : 79.70 µM to α-pinene and 66.23 µM to 1,8-cineole) and cell morphology, inhibiting ROS production (the most active compound was 1,8-cineole by decreasing the ROS production over 30-45% at 10 and 25 μM) and lipid peroxidation and increasing the endogenous antioxidant status (glutathione levels and CAT, SOD, GR, GPx, and HO-1 activity and protein expression).
These findings demonstrate for the first time the effects of the monoterpenes 1,8-cineole and α-pinene identified in S. lavandulifolia essential oil as regulators of cellular redox balance in astrocytes.
熏衣草叶鼠尾草已被用于民间医学治疗记忆和痴呆问题。该物种含有多种生物活性萜类化合物，这可能是其有效性的原因。
分析熏衣草叶鼠尾草精油的成分，并研究其主要化合物α-蒎烯和1,8-桉叶素对U373-MG细胞系中H2O2诱导的氧化应激的体外潜在细胞保护和抗氧化活性。
通过气相色谱分析化学成分；使用ORAC法测定抗氧化能力，使用MTT法（用于细胞活力）（浓度范围：10-400μM）、DCFH-DA法（用于细胞内活性氧生成）、硫代巴比妥酸反应物质（TBARS）法（用于脂质过氧化）以及分光光度技术和蛋白质印迹法（分别用于酶活性和蛋白质表达）在10和25μM下评估细胞保护活性。
α-蒎烯（18.39%）和1,8-桉叶素（19.57%）被鉴定为熏衣草叶鼠尾草精油中的主要化合物。用这些单萜预处理可保护U373-MG细胞免受H2O2诱导的氧化损伤，减轻细胞活力丧失（α-蒎烯的IC50为79.70μM，1,8-桉叶素为66.23μM）和细胞形态变化，抑制活性氧生成（最具活性的化合物是1,8-桉叶素，在10和25μM时可使活性氧生成减少30-45%）和脂质过氧化，并提高内源性抗氧化状态（谷胱甘肽水平以及CAT、SOD、GR、GPx和HO-1的活性和蛋白质表达）。
这些发现首次证明了熏衣草叶鼠尾草精油中鉴定出的单萜1,8-桉叶素和α-蒎烯作为星形胶质细胞中细胞氧化还原平衡调节剂的作用。
