Döğen Aylin, Gümral Ramazan, İlkit Macit
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Mersin, Mersin, Turkey.
Mycoses. 2015 Jan;58(1):40-7. doi: 10.1111/myc.12269. Epub 2014 Dec 5.
Dermatophytes are some of the most common fungal pathogens in both humans and animals. These fungi release enzymes (e.g., keratinases) that play roles in their pathogenesis. Little is known about their haemolytic and co-haemolytic (CAMP-like) activities; however, in bacteria, these components play significant roles in pathogenesis. This study characterised these two factors in 45 dermatophyte strains (representing the genera Arthroderma, Epidermophyton, Microsporum and Trichophyton) using Columbia agar (CA) supplemented with 5% bovine, ovine and equine erythrocytes. Haemolysis was best observed on CA supplemented with ovine erythrocytes followed by equine and bovine erythrocytes, while CAMP-like reactions occurred using bovine and ovine but not equine erythrocytes. Haemolytic and CAMP-like activities were best observed using ovine and bovine erythrocytes in CA in 44 and 38 strains at 7 and 3 days respectively. Most dermatophytes recovered from both symptomatic and asymptomatic lesions had haemolytic and CAMP-like activities. We suggest that the haemolytic and CAMP-like activities are not correlated with ecological characteristics, isolation sites or clinical manifestations of dermatophytic fungi. We also believe that this study has the potential to contribute to the existing literature on dermatophytes and dermatophyte pathogenesis.
皮肤癣菌是人和动物中最常见的真菌病原体之一。这些真菌会释放一些酶（如角蛋白酶），这些酶在其致病过程中发挥作用。关于它们的溶血活性和协同溶血（类CAMP）活性，人们了解甚少；然而，在细菌中，这些成分在致病过程中起着重要作用。本研究使用添加了5%牛、羊和马红细胞的哥伦比亚琼脂（CA）对45株皮肤癣菌菌株（代表节皮菌属、表皮癣菌属、小孢子菌属和毛癣菌属）的这两种因素进行了表征。在添加羊红细胞的CA上观察到的溶血效果最佳，其次是马和牛红细胞，而使用牛和羊红细胞会出现类CAMP反应，使用马红细胞则不会。在添加羊和牛红细胞的CA上，分别在第7天和第3天对44株和38株菌株观察到了最佳的溶血和类CAMP活性。从有症状和无症状病变中分离出的大多数皮肤癣菌都具有溶血和类CAMP活性。我们认为，溶血和类CAMP活性与皮肤癣菌的生态特征、分离部位或临床表现无关。我们还相信，这项研究有可能为现有的关于皮肤癣菌和皮肤癣菌致病机制的文献做出贡献。
