Evaluation of CAMP-Like Effect, Biofilm Formation, and Discrimination of from Vaginal Species.

a species recovered from female genital specimens is highly close to . The present study was conducted to discriminate from presumptive vaginal strains by molecular assay and evaluate their hemolysin activity, biofilm formation, and cohemolytic effect (CAMP) with vaginal bacterial flora. A total of 110 stock vaginal isolates were examined by gene amplification. Hemolysin activity and the ability of biofilm formation were evaluated by blood plate assay and visual detection methods, respectively. , , and were used to evaluate the CAMP-like effects in Sabouraud blood agar media. Based on the size of the amplicons (941 bp), all isolates were identified as . All samples were able to produce beta-hemolysin. Moreover, 69 out of 110 of the isolates (62.7%) were biofilm-positive, 54 out of 110 isolates (49%) demonstrated cohemolytic effects with , and 48 out of 110 showed this effect with (43.6%). All isolates were CAMP-negative with . We detected all isolates as and almost half of the isolates were CAMP-positive with and , suggesting that these bacteria increase the pathogenicity of in vaginal candidiasis.