Holte H, Blomhoff H K, Beiske K, Funderud S, Torjesen P, Gaudernack G, Stokke T, Smeland E B
Laboratory for Immunology, Institute for Cancer Research, Oslo, Norway.
Eur J Immunol. 1989 Jul;19(7):1221-5. doi: 10.1002/eji.1830190711.
We have investigated several aspects of the inhibitory effects of monoclonal antibodies (mAb) directed against MHC class II antigens in B cell activation/proliferation, using a panel of mAb specifically reactive with antigens encoded by HLA class II loci (DP, DQ, DR). All mAb except the anti-DP mAb inhibited significantly anti-mu plus B cell growth factor-induced DNA synthesis. Only one mAb, however, which was reactive with gene products of all three class II loci (DP, DQ, DR) inhibited anti-mu-induced DNA synthesis as well as c-myc mRNA expression. In addition, the same mAb inhibited the early events induced by anti-mu stimulation alone, including phosphatidylinositol turnover and elevation of [Ca2+]i. In contrast to previous findings in the murine system, none of the anti-MHC class II mAb used in this study increased the cAMP levels.
我们使用一组与HLA II类基因座（DP、DQ、DR）编码的抗原特异性反应的单克隆抗体（mAb），研究了针对MHC II类抗原的单克隆抗体在B细胞激活/增殖中的抑制作用的几个方面。除抗DP单克隆抗体外，所有单克隆抗体均显著抑制抗μ加B细胞生长因子诱导的DNA合成。然而，只有一种与所有三个II类基因座（DP、DQ、DR）的基因产物反应的单克隆抗体抑制抗μ诱导的DNA合成以及c-myc mRNA表达。此外，相同的单克隆抗体抑制单独抗μ刺激诱导的早期事件，包括磷脂酰肌醇代谢和细胞内钙离子浓度升高。与先前在小鼠系统中的发现相反，本研究中使用的抗MHC II类单克隆抗体均未提高cAMP水平。
