Pickard S, Shankar G, Burnham K
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater 74078.
Immunology. 1994 Dec;83(4):568-72.
Superantigens were examined for effects on the distribution of Langerhans' cells (LC) in mouse skin. This was accomplished by analysing the expression of LC-specific markers, ATPase and IA among the epidermal portion of cultured sections of mouse skin following treatment with staphylococcal enterotoxins. In this study, treatment of skin sections with staphylococcal enterotoxin A or exfoliative toxin but not toxic shock syndrome toxin led to significant depletion of LC. This depletion was inhibited by agents which specifically block the action of GTP binding proteins or their associated kinases (cholera and pertussis toxins and H-8) as well as those which block protein or RNA synthesis. Therefore, signals which lead to LC depletion in response to staphylococcal enterotoxins appear to involve a cholera and pertussis toxin-sensitive GTP-binding protein and protein synthesis. These requirements are identical to those observed previously for LC depletion following exposure of skin to ultraviolet radiation.
研究了超抗原对小鼠皮肤中朗格汉斯细胞（LC）分布的影响。这是通过分析用葡萄球菌肠毒素处理后的小鼠皮肤培养切片表皮部分中LC特异性标志物ATP酶和IA的表达来实现的。在本研究中，用葡萄球菌肠毒素A或剥脱毒素而非中毒性休克综合征毒素处理皮肤切片，导致LC显著减少。这种减少受到特异性阻断GTP结合蛋白或其相关激酶作用的试剂（霍乱毒素和百日咳毒素以及H-8）以及阻断蛋白质或RNA合成的试剂的抑制。因此，响应葡萄球菌肠毒素导致LC减少的信号似乎涉及一种对霍乱毒素和百日咳毒素敏感的GTP结合蛋白和蛋白质合成。这些要求与之前观察到的皮肤暴露于紫外线辐射后LC减少的要求相同。
