Eltzov Evgeni, Slobodnik Vladimir, Ionescu Rodica E, Marks Robert S
Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; School of Material Science and Engineering, Nanyang Technology University, Nanyang Avenue, 639798, Singapore; CREATE, Singapore.
Department of Biotechnology Engineering, Faculty of Engineering Science, Sami Shimon, Ashdod, Israel.
Talanta. 2015 Jan;132:583-90. doi: 10.1016/j.talanta.2014.09.032. Epub 2014 Oct 13.
Potential threat on drinking water requires monitoring solutions, such as the one proposed herein, as a real-time, wide ranged, water monitoring system to detect the presence of toxicants in water. We studied the role of a selected number of parameters affecting performance and, thus, improved the prototype into an optimized next-generation device, resulting in enabling increased measurement duration, coupled with increased sensitivity. The chosen parameters in question were the peristaltic flow system, the fiber probe matrix stability through a re-design of the fiber probe holder and flow unit cell, as well as the modulation of bacterial medium concentration to increase bioreporter performance while keeping biofouling in check. Measurements were made with spiked samples and validated with polluted field-collected samples.
饮用水面临的潜在威胁需要监测解决方案，比如本文提出的作为一种实时、大范围的水监测系统，以检测水中有毒物质的存在。我们研究了若干影响性能的参数的作用，从而将原型改进为优化的下一代设备，实现了延长测量持续时间并提高灵敏度。所涉及的选定参数包括蠕动流系统、通过重新设计光纤探头支架和流动单元来提高光纤探头矩阵的稳定性，以及调节细菌培养基浓度以提高生物报告器性能同时控制生物污垢。使用加标样品进行测量，并通过现场采集的污染样品进行验证。
