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用于监测水体毒性的生物发光生物报告垫生物传感器。

Bioluminescent bioreporter pad biosensor for monitoring water toxicity.

作者信息

Axelrod Tim, Eltzov Evgeni, Marks Robert S

机构信息

Department of Biotechnology Engineering, Faculty of Engineering Science, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Department of Biotechnology Engineering, Faculty of Engineering Science, Ben-Gurion University of the Negev, Beer-Sheva, Israel; School of Material Science and Engineering, Nanyang Technology University, Nanyang Avenue, 639798 Singapore.

出版信息

Talanta. 2016;149:290-297. doi: 10.1016/j.talanta.2015.11.067. Epub 2015 Nov 28.

Abstract

Toxicants in water sources are of concern. We developed a tool that is affordable and easy-to-use for monitoring toxicity in water. It is a biosensor composed of disposable bioreporter pads (calcium alginate matrix with immobilized bacteria) and a non-disposable CMOS photodetector. Various parameters to enhance the sensor's signal have been tested, including the effect of alginate and bacterium concentrations. The effect of various toxicants, as well as, environmental samples were tested by evaluating their effect on bacterial luminescence. This is the first step in the creation of a sensitive and simple operative tool that may be used in different environments.

摘要

水源中的有毒物质令人担忧。我们开发了一种价格实惠且易于使用的工具,用于监测水中的毒性。它是一种生物传感器,由一次性生物报告垫(固定有细菌的海藻酸钙基质)和非一次性互补金属氧化物半导体光电探测器组成。已经测试了各种增强传感器信号的参数,包括海藻酸盐和细菌浓度的影响。通过评估各种有毒物质以及环境样品对细菌发光的影响来进行测试。这是创建一种可在不同环境中使用的灵敏且简单的操作工具的第一步。

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