文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Takenaka A, Tayama T, Takahashi S I, Kato H, Noguchi T, Naito H

Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.

Int J Biochem. 1989;21(5):497-508. doi: 10.1016/0020-711x(89)90129-8.

A method to microinject proteins into cells through packaging proteins to erythrocyte ghosts (erythrocyte-mediated microinjection) was modified partially in order to apply the method to primary cultures of rat hepatocytes. 2. Degradation of the microinjected proteins was examined employing the improved method. The mean half-life of the injected endogenous liver protein was 20 hr. The data suggested that the injected proteins are degraded through both lysosomal and non-lysosomal proteolytic pathways probably depending on their structure. 3. The present method to microinject exogenous proteins into primary cultures of rat hepatocytes can be employed usefully for the investigations of protein metabolism in liver.

为了将通过将蛋白质包装到红细胞血影中来向细胞中微量注射蛋白质的方法（红细胞介导的微量注射）应用于大鼠肝细胞原代培养，对该方法进行了部分改进。2. 采用改进后的方法检测了微量注射蛋白质的降解情况。注射的内源性肝脏蛋白质的平均半衰期为20小时。数据表明，注射的蛋白质可能根据其结构通过溶酶体和非溶酶体蛋白水解途径降解。3. 目前这种向大鼠肝细胞原代培养物中微量注射外源蛋白质的方法可有效地用于肝脏蛋白质代谢的研究。

Takenaka A, Tayama T, Takahashi S I, Kato H, Noguchi T, Naito H

Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.

Int J Biochem. 1989;21(5):497-508. doi: 10.1016/0020-711x(89)90129-8.

A method to microinject proteins into cells through packaging proteins to erythrocyte ghosts (erythrocyte-mediated microinjection) was modified partially in order to apply the method to primary cultures of rat hepatocytes. 2. Degradation of the microinjected proteins was examined employing the improved method. The mean half-life of the injected endogenous liver protein was 20 hr. The data suggested that the injected proteins are degraded through both lysosomal and non-lysosomal proteolytic pathways probably depending on their structure. 3. The present method to microinject exogenous proteins into primary cultures of rat hepatocytes can be employed usefully for the investigations of protein metabolism in liver.

为了将通过将蛋白质包装到红细胞血影中来向细胞中微量注射蛋白质的方法（红细胞介导的微量注射）应用于大鼠肝细胞原代培养，对该方法进行了部分改进。2. 采用改进后的方法检测了微量注射蛋白质的降解情况。注射的内源性肝脏蛋白质的平均半衰期为20小时。数据表明，注射的蛋白质可能根据其结构通过溶酶体和非溶酶体蛋白水解途径降解。3. 目前这种向大鼠肝细胞原代培养物中微量注射外源蛋白质的方法可有效地用于肝脏蛋白质代谢的研究。