Nguyen P, Shusterman R D, Barnett R B, Ishihara Y, Gottlieb J E, Albertine K H, Gee M H, Peters S P
Department of Medicine, Jefferson Medical College, Philadelphia, Pa.
Int Arch Allergy Appl Immunol. 1989;89(2-3):301-5. doi: 10.1159/000234964.
The role played by neutrophils (PMNs) in the genesis of lung injury in diverse clinical situations, such as bronchial asthma, idiopathic pulmonary fibrosis, and the adult respiratory distress syndrome, is an area of intensive investigation. Functional studies of PMNs, particularly those obtained from the alveoli by bronchoalveolar lavage, should shed light on their contribution to lung injury. However, it has not been demonstrated whether procedures used to harvest cells from the lung (bronchoalveolar lavage), particularly the potentially prolonged exposure to saline, commonly used to perform lavage, and other components of lavage fluid, can alter the functional characteristics of PMNs. In this report we demonstrate that a 2- to 3-hour exposure of neutrophils to saline from both humans and sheep in vitro does not alter the functional characteristics of PMNs as determined by superoxide anion generation after activation with phorbol myristate acetate (PMA; 6.96 +/- 0.44 vs. 7.60 +/- 0.32 nmol O2-/250,000 PMNs for control and saline-treated human cells, respectively, after a 45-min incubation with 10(-7) M PMA, and 4.73 +/- 0.30 vs. 4.50 +/- 0.42 nmol O2-/250,000 PMNs for control and saline-treated sheep cells). In a second series of experiments, we studied the effect of exposure of human PMNs to bronchoalveolar lavage fluid supernatants obtained from normal volunteers on superoxide anion generation by neutrophils.(ABSTRACT TRUNCATED AT 250 WORDS)
中性粒细胞（PMNs）在多种临床情况下（如支气管哮喘、特发性肺纤维化和成人呼吸窘迫综合征）肺损伤发生过程中所起的作用，是一个深入研究的领域。对PMNs的功能研究，尤其是通过支气管肺泡灌洗从肺泡中获取的PMNs的功能研究，应能阐明它们对肺损伤的作用。然而，用于从肺中采集细胞的程序（支气管肺泡灌洗），特别是通常用于进行灌洗的生理盐水的潜在长时间暴露以及灌洗流体的其他成分，是否会改变PMNs的功能特性，尚未得到证实。在本报告中，我们证明，体外将中性粒细胞暴露于人和绵羊的生理盐水2至3小时，不会改变用佛波酯肉豆蔻酸酯（PMA）激活后通过超氧阴离子生成所测定的PMNs的功能特性（分别在与10(-7)M PMA孵育45分钟后，对照和生理盐水处理的人细胞中，超氧阴离子生成量分别为6.96 +/- 0.44与7.60 +/- 0.32 nmol O2-/250,000个PMNs，对照和生理盐水处理的绵羊细胞中分别为4.73 +/- 0.30与4.50 +/- 0.42 nmol O2-/250,000个PMNs）。在第二系列实验中，我们研究了将人PMNs暴露于从正常志愿者获得的支气管肺泡灌洗流体上清液对中性粒细胞超氧阴离子生成的影响。（摘要截断于250字）
