Süle Judit, Kõrösi Tímea, Hucker Attila, Varga László
Institute of Food Science Faculty of Agricultural and Food Sciences University of West Hungary Mosonmagyaróvár Hungary Institute of Food Science, Faculty of Agricultural and Food Sciences, University of West Hungary, Mosonmagyaróvár, Hungary.
Hungarian Dairy Research Institute Mosonmagyaróvár Hungary Hungarian Dairy Research Institute, Mosonmagyaróvár, Hungary.
Braz J Microbiol. 2014 Oct 9;45(3):1023-30. doi: 10.1590/s1517-83822014000300035. eCollection 2014.
The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumeration of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus, however, it also promoted the growth of Lb. casei strains. For this reason, MRS-CC agar can only be used as a selective medium for the enumeration of Lb. acidophilus if Lb. casei is not present in a product at levels similar to or exceeding those of Lb. acidophilus. Unlike bifidobacteria and coccus-shaped LAB, all the lactobacilli strains involved in this work were found to grow well in MRS pH 5.4 agar incubated under anaerobiosis at 37 °C for 72 h. Therefore, this method proved to be particularly suitable for the selective enumeration of Lactobacillus spp.
本研究的目的是测试转半乳糖基寡糖-莫匹罗星锂盐（TOS-MUP）琼脂和MRS-克林霉素-环丙沙星（MRS-CC）琼脂以及其他几种培养基，用于选择性计数常用于制作发酵乳的双歧杆菌和乳酸菌（LAB）种类的适用性。对总共13株属于8个种和5个属的乳杆菌纯培养悬浮液在各种培养条件下的生长能力进行了测试。TOS-MUP琼脂成功用于动物双歧杆菌乳酸亚种BB-12和短双歧杆菌M-16 V的选择性计数。MRS-CC琼脂对嗜酸乳杆菌显示出相对较好的选择性，然而，它也促进了干酪乳杆菌菌株的生长。因此，如果产品中干酪乳杆菌的含量不与嗜酸乳杆菌相当或超过嗜酸乳杆菌的含量，MRS-CC琼脂只能用作嗜酸乳杆菌计数的选择性培养基。与双歧杆菌和球菌形LAB不同，本研究中涉及的所有乳杆菌菌株在37℃厌氧培养72小时的MRS pH 5.4琼脂中生长良好。因此，该方法被证明特别适合于乳杆菌属的选择性计数。
