Hamouda Ragaa A, Makharita Rabab R, Qarabai Fauzia A K, Shahabuddin Fathi S, Saddiq Amna A, Bahammam Laila Ahmed, El-Far Shaymaa W, Bukhari Mamdouh A, Elaidarous Mohammad A, Abdella Asmaa
Department of Biology, College of Sciences and Arts at Khulis, University of Jeddah, Jeddah 21959, Saudi Arabia.
Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City 32897, Egypt.
Microorganisms. 2023 Dec 19;12(1):1. doi: 10.3390/microorganisms12010001.
Dental caries is an infectious oral disease caused by the presence of different bacteria in biofilms. Multidrug resistance (MDR) is a major challenge of dental caries treatment. Swabs were taken from 65 patients with dental caries in Makkah, Saudi Arabia. Swabs were cultivated on mitis salivarius agar and de Man, Rogosa, and Sharpe (MRS) agar. VITEK 2 was used for the identification of isolated bacteria. Antibiotic susceptibility testing of the isolated bacteria was performed using commercial antibiotic disks. Ulva lactuca was used as a reducing agent and cellulose source to create nanocellulose and Ag/cellulose nanocomposites. Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction spectroscopy (XRD) were used to characterize nanocellulose and Ag/cellulose nanocomposites. The results showed that most bacterial isolates were spp., followed by spp. on mitis salivarius media. spp. and group f-1 were the bacterial isolates on de Man, Rogosa, and Sharpe (MRS) media. The antibiotic susceptibility test revealed resistance rates of 77%, 93%, 0, 83%, 79%, and 79% against penicillin G, Augmentin, metronidazole, ampicillin, ciprofloxacin, and cotrimoxazole, respectively. Ag/cellulose nanocomposites and Ag/cellulose nanocomposites with fluoride were the most effective antibacterial agents. The aim of this work was to assess the antibacterial activity of Ag/cellulose nanocomposites with and without fluoride against bacteria isolated from the oral cavities of patients with dental caries. This study demonstrated that Ag/cellulose nanocomposites have antibacterial properties against multidrug-resistant bacteria that cause dental caries.
龋齿是由生物膜中存在的不同细菌引起的一种传染性口腔疾病。多重耐药性（MDR）是龋齿治疗的一项重大挑战。从沙特阿拉伯麦加的65名龋齿患者中采集了拭子。将拭子接种在唾液链球菌琼脂和德氏、罗氏和夏普（MRS）琼脂上培养。使用VITEK 2鉴定分离出的细菌。使用商业抗生素纸片对分离出的细菌进行药敏试验。石莼被用作还原剂和纤维素来源以制备纳米纤维素和银/纤维素纳米复合材料。采用傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDS）和X射线衍射光谱（XRD）对纳米纤维素和银/纤维素纳米复合材料进行表征。结果表明，在唾液链球菌培养基上，大多数细菌分离株为 菌属，其次是 菌属。在德氏、罗氏和夏普（MRS）培养基上的细菌分离株为 菌属和f-1菌群。药敏试验显示，对青霉素G、阿莫西林克拉维酸、甲硝唑、氨苄西林、环丙沙星和复方新诺明的耐药率分别为77%、93%、0、83%、79%和79%。含氟和不含氟的银/纤维素纳米复合材料是最有效的抗菌剂。这项工作的目的是评估含氟和不含氟的银/纤维素纳米复合材料对从龋齿患者口腔中分离出的细菌的抗菌活性。本研究表明，银/纤维素纳米复合材料对引起龋齿的多重耐药细菌具有抗菌特性。
