Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon 200-701, Republic of Korea ; Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon 200-701, Republic of Korea.
Evid Based Complement Alternat Med. 2014;2014:646039. doi: 10.1155/2014/646039. Epub 2014 Nov 16.
Caffeic acid phenethyl ester (CAPE) is an active component of propolis that has a variety of potential pharmacological effects. Although we previously demonstrated that propolis has antidepressant-like activity, the effect of CAPE on this activity remains unknown. The present study assessed whether treatment with CAPE (5, 10, and 20 µmol/kg for 21 days) has an antidepressant-like effect in mice subjected to chronic unpredictable stress via tail suspension (TST) and forced swim (FST) tests. CAPE administration induced behaviors consistent with an antidepressant effect, evidenced by decreased immobility in the TST and FST independent of any effect on serum corticosterone secretion. Western blots, conducted subsequent to behavioral assessment, revealed that CAPE significantly decreased glucocorticoid receptor phosphorylation at S234 (pGR(S234)), resulting in an increased pGR(S220/S234) ratio. We also observed negative correlations between pGR(S220)/(S234) and p38 mitogen-activated protein kinase (p38MAPK) phosphorylation, which was decreased by CAPE treatment. These findings suggest that CAPE treatment exerts an antidepressant-like effect via downregulation of p38MAPK phosphorylation, thereby contributing to enhanced GR function.
咖啡酸苯乙酯（CAPE）是蜂胶的一种活性成分，具有多种潜在的药理作用。虽然我们之前已经证明蜂胶具有抗抑郁样活性，但 CAPE 对这种活性的影响尚不清楚。本研究评估了 CAPE（5、10 和 20μmol/kg，连续 21 天）治疗是否通过悬尾（TST）和强迫游泳（FST）试验对慢性不可预测应激小鼠具有抗抑郁样作用。CAPE 给药诱导与抗抑郁作用一致的行为，这表现在 TST 和 FST 中的不动时间减少，而对血清皮质酮分泌没有任何影响。行为评估后进行的 Western blot 显示，CAPE 显著降低了糖皮质激素受体在 S234 处的磷酸化（pGR(S234)），导致 pGR(S220/S234) 比值增加。我们还观察到 pGR(S220)/(S234) 与 p38 丝裂原活化蛋白激酶（p38MAPK）磷酸化之间存在负相关，CAPE 处理可降低 p38MAPK 磷酸化。这些发现表明，CAPE 治疗通过下调 p38MAPK 磷酸化发挥抗抑郁样作用，从而增强 GR 功能。
