Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Cell Stem Cell. 2014 Dec 4;15(6):687-91. doi: 10.1016/j.stem.2014.10.018.
Neural cells can be derived either from pluripotent or adult stem cells via differentiation or by transdifferentiation from other cell types with the aid of tissue regulators. We compared the chromosomal stability of over 500 neural cell samples from human and mouse with virtual karyotyping (e-karyotyping). We detected notable genomic instability in cells derived from pluripotent or adult stem cells, but surprisingly, transdifferentiated cells seemed more chromosomally stable, except if they were reprogrammed using pluripotency factors.
神经细胞可以通过分化从多能干细胞或成体干细胞中获得，或者在组织调节剂的辅助下由其他细胞类型转分化而来。我们通过虚拟核型分析（e 核型分析）比较了来自人和小鼠的 500 多个神经细胞样本的染色体稳定性。我们在多能干细胞或成体干细胞来源的细胞中检测到明显的基因组不稳定性，但令人惊讶的是，转分化细胞似乎具有更高的染色体稳定性，除非它们使用多能性因子进行重编程。
