Ossenkoppele G J, Wijermans P W, Nauta J J, Huijgens P C, Langenhuijsen M M
Department of Haematology, Free University Hospital, Amsterdam, The Netherlands.
Leuk Res. 1989;13(7):609-14. doi: 10.1016/0145-2126(89)90129-x.
From ten consecutive patients with acute myeloid leukemia leukemic cells were isolated and cultured with and without 10(-6), 10(-7) and 10(-8) M 1.25(OH)2D3 (vit D3), retinoic acid (RA) cytosine-arabinoside (ARA-C) and 1.0, 1.25 and 1.5% dimethyl sulfoxide (DMSO). Maturation was measured with a comprehensive panel of qualitative and quantitative parameters of maturation. Six of those ten leukemias showed significant (p less than 0.01) changes in at least three parameters after exposure to either one of the differentiation inducers. Vit D3 induced maturation in four leukemias, in three of them clearly in monocytic direction. ARA-C showed changes in one leukemia in only three parameters not pointing to either granulocytic or monocytic direction. Maturation in granulocytic direction was observed after exposure to RA in one leukemia. Maturation induction was observed in six out of ten freshly isolated leukemic cells with vit D3 being the most potent inducer of maturation in monocytic direction. The data about inducibility of maturation in freshly isolated human leukemic cells are reviewed and discussed.
从十名连续的急性髓系白血病患者中分离出白血病细胞，并在添加和不添加10(-6)、10(-7)和10(-8)M 1,25(OH)2D3（维生素D3）、维甲酸（RA）、阿糖胞苷（ARA-C）以及1.0%、1.25%和1.5%二甲基亚砜（DMSO）的情况下进行培养。通过一组全面的定性和定量成熟参数来测量成熟度。这十例白血病中有六例在暴露于任何一种分化诱导剂后，至少三个参数出现了显著（p小于0.01）变化。维生素D3诱导了四例白血病的成熟，其中三例明显向单核细胞方向成熟。阿糖胞苷仅在一例白血病的三个参数中显示出变化，未指向粒细胞或单核细胞方向。在一例白血病中，暴露于维甲酸后观察到向粒细胞方向的成熟。在十例新鲜分离的白血病细胞中，有六例观察到成熟诱导，维生素D3是单核细胞方向最有效的成熟诱导剂。本文对新鲜分离的人白血病细胞中成熟诱导性的数据进行了综述和讨论。
