Bani D, Bani Sacchi T, Biliotti G
Department of Human Anatomy and Histology, University of Florence, Italy.
Pancreas. 1989;4(4):492-5. doi: 10.1097/00006676-198908000-00015.
In this study, liver metastases from a patient with a pancreatic glucagonoma producing the syndrome have been investigated histologically, ultrastructurally, and immunocytochemically. A comparison has also been made between the metastases and the primary pancreatic tumor investigated in a parallel study. In the metastatic tissue, glucagon-, pancreatic polypeptide (PP)-, and somatostatin-containing cells were found together with a majority of cells without any immunoreactivity. Glucagon-positive cells were much more numerous than PP- and somatostatin-immunoreactive cells. As in the primary tumor, double immunogold staining of ultrathin sections demonstrated the co-existence of glucagon and PP immunoreactivities in most of the granulated cells, but PP immunolabeling was often faint, so that it probably could not be revealed by the PAP method in light microscopical sections. Such a finding, together with the histological and ultrastructural features, is consistent with an ontogenic and phylogenetic primitiveness of the metastatic cell population.
在本研究中，对一名患有产生该综合征的胰腺高血糖素瘤患者的肝转移灶进行了组织学、超微结构和免疫细胞化学研究。同时，还将这些转移灶与在一项平行研究中所研究的原发性胰腺肿瘤进行了比较。在转移组织中，发现了含有胰高血糖素、胰多肽（PP）和生长抑素的细胞，同时大多数细胞没有任何免疫反应性。胰高血糖素阳性细胞比PP和生长抑素免疫反应性细胞多得多。与原发性肿瘤一样，超薄切片的双重免疫金染色显示大多数颗粒细胞中胰高血糖素和PP免疫反应性共存，但PP免疫标记通常很微弱，因此在光学显微镜切片中可能无法用PAP法显示。这一发现与组织学和超微结构特征一起，与转移细胞群体的个体发生和系统发育原始性一致。
