Sayed N I, Rupert C S
Photochem Photobiol. 1989 Jul;50(1):153-6. doi: 10.1111/j.1751-1097.1989.tb04141.x.
Exponentially growing cells of the PtK-2 line (ATCC No. CCL56, from the marsupial Potorous tridactylus) require protein and RNA synthesis in a limited period following UV-radiation damage for optimal recovery as colony formers [Overberg et al. (1988) Mutat. Res. 194, 83-92]. Overall behavior suggests the operation of damage-induced recovery processes. The capacity of confluent cell monolayers for infection with unirradiated herpes simplex virus 1 (HSV-1) is sharply reduced by UV-irradiation. We have followed capacity changes in exponentially growing cells after irradiation and varying amounts of photoreactivation by means of an infectious center assay. These changes closely parallel changes of colony formation. Spontaneous recovery of capacity in the dark occurs over approximately the same time period that the UV sensitivity of colony formation depends on macromolecular synthesis. The effect of photoreactivation is complementary rather than additive to this recovery, suggesting that the dark recovery in this period concerns pyrimidine dimers in cell DNA.
PtK - 2细胞系（ATCC编号CCL56，源自有袋动物三趾袋狸）处于指数生长期的细胞在紫外线辐射损伤后的有限时间内需要蛋白质和RNA合成，以实现作为集落形成细胞的最佳恢复[奥弗贝格等人（1988年），《突变研究》194卷，83 - 92页]。总体行为表明存在损伤诱导的恢复过程。紫外线辐射会使汇合的细胞单层感染未辐照单纯疱疹病毒1（HSV - 1）的能力大幅降低。我们通过感染中心测定法跟踪了辐照后及不同程度光复活处理的指数生长期细胞的能力变化。这些变化与集落形成的变化密切平行。在黑暗中能力的自发恢复发生在与集落形成的紫外线敏感性依赖于大分子合成大致相同的时间段内。光复活的作用是对这种恢复起到补充而非累加作用，这表明在此期间的黑暗恢复涉及细胞DNA中的嘧啶二聚体。
