Hoy C A, Rupert C S
Mutat Res. 1984 Aug;140(4):199-203. doi: 10.1016/0165-7992(84)90077-0.
Colony-forming ability of Potorous tridactylus-kidney (PtK-2) cells was measured after exposure to 254-nm ultraviolet (UV) light and cycloheximide. Addition of 5 microM cycloheximide after exposure of the cells to UV light greatly decreased cell survival. Maximum effect of the drug was obtained by 24-h exposure after irradiation. The cycloheximide sensitivity of irradiated cells was eliminated if addition of the drug was delayed for 8-10 h after irradiation, or if the cells received photoreactivating light treatment prior to cycloheximide exposure. Thus, a major component of pyrimidine dimer removal may involve a cycloheximide-sensitive mechanism.
在将三趾袋狸肾（PtK - 2）细胞暴露于254纳米紫外线（UV）和放线菌酮后，测定了其集落形成能力。在细胞暴露于紫外线后添加5微摩尔的放线菌酮会大大降低细胞存活率。在照射后24小时的暴露可获得该药物的最大效果。如果在照射后8 - 10小时延迟添加药物，或者如果细胞在放线菌酮暴露之前接受光复活光处理，则可消除照射细胞对放线菌酮的敏感性。因此，嘧啶二聚体去除的一个主要成分可能涉及一种对放线菌酮敏感的机制。
