Zhang Q, Wang X N, Gao E W, Cheng C P
Sheng Li Xue Bao. 1989 Feb;41(1):70-5.
The ovaries taken from the immature female rats primed with PMSG-hCG were digested with collagenase-DNAase solution to obtain the luteal cell suspensions. Luteal cells were then incubated with different doses of hCG, cAMP or progesterone for 1.5 hours. The contents of tyrosine in luteal cell suspensions were determined by thin layer chromatogram using dual-wavelength chromatogram scanner. It was found that the content of tyrosine in rat luteal cell suspensions was 1.41 + 0.24 nmol/L/10(6) cells after one hour incubation. hCG, cAMP and progesterone all could significantly increase the content of tyrosine in the suspensions, suggesting the release of "endogenous tyrosine". The increase of tyrosine was not due to increased transformation of phenylalanine, the precursor of tyrosine, and increased protein synthesis, because both phenylalanine and cycloheximide failed to influence such increase.
从用孕马血清促性腺激素-人绒毛膜促性腺激素预处理的未成熟雌性大鼠中取出卵巢，用胶原酶-DNA酶溶液消化以获得黄体细胞悬液。然后将黄体细胞与不同剂量的人绒毛膜促性腺激素、环磷酸腺苷或孕酮孵育1.5小时。使用双波长色谱扫描仪通过薄层色谱法测定黄体细胞悬液中酪氨酸的含量。发现孵育1小时后，大鼠黄体细胞悬液中酪氨酸的含量为1.41 + 0.24 nmol/L/10(6)个细胞。人绒毛膜促性腺激素、环磷酸腺苷和孕酮均能显著增加悬液中酪氨酸的含量，提示“内源性酪氨酸”的释放。酪氨酸的增加并非由于酪氨酸前体苯丙氨酸转化增加以及蛋白质合成增加，因为苯丙氨酸和环己酰亚胺均未能影响这种增加。
