Kumar A, Das M, Manchanda S K, Gauri K K
Exp Clin Endocrinol. 1987 Mar;89(1):1-6. doi: 10.1055/s-0029-1210620.
The study was carried out to investigate whether the luteal steroidogenesis in response to tropic stimulus is mediated through de novo synthesis of protein(s). Luteal cell suspension was prepared by collagenase-DNAse treatment of the bovine corpora lutea, weighing 4-6 g. Cells equivalent to 250 micrograms of protein were incubated in a total volume of 550 microliter of medium 199 with or without various test substances. Production of progesterone by the luteal cells was stimulated by hCG in a dose dependent manner. Incorporation of 3H-leucine into the cellular proteins was concomitantly enhanced. Emetine, cycloheximide and puromycin inhibited both basal and hCG stimulated protein synthesis as well as progesterone production in the cells. Thus, luteal steroidogenesis induced by hCG seems to be dependent upon de novo synthesis of a protein(s).
本研究旨在探讨黄体对促性腺刺激的类固醇生成是否通过蛋白质的从头合成介导。通过用胶原酶-DNA酶处理4-6克重的牛黄体来制备黄体细胞悬液。将相当于250微克蛋白质的细胞在550微升含或不含各种测试物质的199培养基中孵育。人绒毛膜促性腺激素（hCG）以剂量依赖性方式刺激黄体细胞产生孕酮。同时，细胞中3H-亮氨酸掺入细胞蛋白的量也增加。依米丁、放线菌酮和嘌呤霉素抑制细胞中的基础和hCG刺激的蛋白质合成以及孕酮产生。因此，hCG诱导的黄体类固醇生成似乎依赖于蛋白质的从头合成。
