原位头颈癌小鼠模型的多模态成像分析及抗CD137肿瘤免疫治疗的应用

Multimodal imaging analysis of an orthotopic head and neck cancer mouse model and application of anti-CD137 tumor immune therapy.

作者信息

Vahle Anne-Kristin, Hermann Sven, Schäfers Michael, Wildner Michael, Kerem Alexander, Öztürk Ender, Jure-Kunkel Maria, Franklin Cindy, Lang Stephan, Brandau Sven

机构信息

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany.

European Institute for Molecular Imaging, University of Münster, Münster, Germany.

出版信息

Head Neck. 2016 Apr;38(4):542-9. doi: 10.1002/hed.23929. Epub 2015 Jun 16.

DOI:10.1002/hed.23929

PMID:25482887

Abstract

BACKGROUND

Recent technical progress makes sophisticated noninvasive imaging methods available for murine models. For the first time, in this study, we applied fluorodeoxyglucose (FDG)-positron emission tomography (PET)-CT and FDG-PET-MRI to a murine orthotopic model of head and neck cancer immunotherapy.

METHODS

Tumor growth of floor of the mouth tumors was evaluated by multimodal small-animal imaging using FDG-PET-CT and FDG-PET-MRI. The immunotherapeutic effects of anti-CD137 antibody therapy were examined on body weight, tumor growth, and tumor-infiltrating immune cells in longitudinal imaging studies and immunohistochemical analyses.

RESULTS

Imaging revealed aggressive, fast-growing tumors without evidence of local or distant metastases. CD137 immunotherapy decreased tumor take and growth and stabilized body weight over time. A clear case of tumor regression was demonstrated by longitudinal PET-CT.

CONCLUSION

The murine model mimics the characteristics of head and neck cancer in humans and offers excellent opportunities to investigate immunomodulatory anticancer drugs. The CD137 antibody showed antitumor effects in some therapy-responsive mice.

摘要