结核分枝杆菌与牛分枝杆菌之间的遗传相关性。

Genetic relatedness among Mycobacterium tuberculosis and M. bovis.

作者信息

Labidi A, Thoen C O

机构信息

University of Texas Health Center, Department of Microbiology, Tyler 75710.

出版信息

Acta Leprol. 1989;7 Suppl 1:217-21.

PMID:2548360

Abstract

Total DNA from two slowly-growing pathogenic mycobacterial species propagated in vitro was isolated, digested with each of 34 restriction endonucleases and analyzed by agarose gel electrophoresis. The most resolved patterns for M. tuberculosis (ATCC 27294) and for M. bovis (ATCC 19210) were obtained respectively using (BamHI, DraI, ClaI, EcoRI, EcoRV, HindIII, HpaI, SalI, SmaI, XbaI and XmaI). The patterns produced for these strains were reproducible and distinguishable from each other respectively using (HindIII, DraI, EcoRI, MboI, Sau3AI and AvaI). However, with several enzymes (SalI, AsuI, Sau96I, MspI and HpaII) the patterns for M. tuberculosis and M. bovis were similar. Evidence was obtained for the presence of dam and dcmI methylations in the DNA of each mycobacterial species respectively using (MboI, Sau3AI, EcoRII, BstNI, Sau96I and AsuI).

摘要

从体外培养的两种生长缓慢的致病性分枝杆菌中分离出总DNA，用34种限制性内切酶分别进行消化，并通过琼脂糖凝胶电泳进行分析。分别使用（BamHI、DraI、ClaI、EcoRI、EcoRV、HindIII、HpaI、SalI、SmaI、XbaI和XmaI）获得了结核分枝杆菌（ATCC 27294）和牛分枝杆菌（ATCC 19210）最清晰的图谱。分别使用（HindIII、DraI、EcoRI、MboI、Sau3AI和AvaI）为这些菌株产生的图谱具有可重复性且彼此可区分。然而，对于几种酶（SalI、AsuI、Sau96I、MspI和HpaII），结核分枝杆菌和牛分枝杆菌的图谱相似。分别使用（MboI、Sau3AI、EcoRII、BstNI、Sau96I和AsuI）获得了证据，证明每种分枝杆菌的DNA中分别存在dam和dcmI甲基化。