结核分枝杆菌和卡介苗菌株代表株的脉冲场凝胶电泳

Pulsed field gel electrophoresis of representatives of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains.

作者信息

Varnerot A, Clément F, Gheorghiu M, Vincent-Lévy-Frébault V

机构信息

Centre National de Référence pour les Mycobactéries, Unité de la Tuberculose et des Mycobactéries, Paris, France.

出版信息

FEMS Microbiol Lett. 1992 Nov 1;77(1-3):155-60. doi: 10.1016/0378-1097(92)90148-h.

DOI:10.1016/0378-1097(92)90148-h

PMID:1459403

Abstract

Using field inversion gel electrophoresis (FIGE), different Mycobacterium tuberculosis strains, such as phage prototypes, exhibit different DNA restriction patterns which are easy to compare. Virulent and avirulent variants of M. tuberculosis H37, as well as daughter strains of M. bovis BCG, display characteristic DNA profiles. BCG strains isolated from suppurative adenitis following vaccination of French patients showed patterns identical to the BCG Pasteur strain used for vaccination. These results demonstrate that FIGE of DNA restriction fragments generated by DraI represents a suitable technique for the analysis of mycobacteria at a genomic level. The DraI profiles allow the differentiation and precise identification of the BCG Pasteur, Glaxo, Russian and Japanese strains.

摘要

使用脉冲场反转凝胶电泳（FIGE），不同的结核分枝杆菌菌株，如噬菌体原型，呈现出易于比较的不同DNA限制性图谱。结核分枝杆菌H37的有毒和无毒变体，以及卡介苗牛型分枝杆菌的子代菌株，都显示出特征性的DNA图谱。从法国患者接种疫苗后发生的化脓性腺炎中分离出的卡介苗菌株，其图谱与用于接种的卡介苗巴斯德菌株相同。这些结果表明，由DraI产生的DNA限制性片段的FIGE是一种在基因组水平分析分枝杆菌的合适技术。DraI图谱能够区分并精确鉴定卡介苗巴斯德、葛兰素、俄罗斯和日本菌株。