Deng Chao, Xu Jingjing, Fu Haitian, Chen Jinghua, Xu Xin
Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Clinical Laboratory, Wuxi No. 4 People's Hospital, Jiangnan University, Wuxi, Jiangsu 214000, P.R. China.
Mol Med Rep. 2015 Apr;11(4):2991-8. doi: 10.3892/mmr.2014.3060. Epub 2014 Dec 8.
The present study described the characterization and biological properties of water‑soluble sulfated polysaccharides prepared from water‑insoluble polysaccharide (DIP), which were extracted from Dictyophora indusiata. The sulfation of DIP was performed using the chlorosulfonic acid‑pyridine method. The water solubilities of the sulfated derivatives were measured at room temperature according to the Chinese Pharmacopoeia. The scavenging activity of hydroxyl radicals and 1,1‑diphenyl‑2‑picrylhydrazyl (DPPH) as determined, together with the reduction ability of the sulfated polysaccharides. The cytotoxic and antiproliferative effects of DIP and the sulfated derivatives on MCF‑7 and B16 cells were then determined using an MTT assay. The substitution degrees of the sulfated polysaccharides were 0.584 (S1‑DIP), 0.989 (S2‑DIP) and 1.549 (S3‑DIP) according to barium chloride‑gelatin nephelometry. Infrared spectroscopy and 13C‑nuclear magnetic resonance indicated that the substitution of S‑DIP occurred mainly at the C‑6 position, followed by the C‑4 and C‑2 positions. A significant increase was noted in the antioxidant activity of the sulfated derivatives compared with that of DIP. In addition, the S‑DIPs exhibited a more marked reducing capacity and clearing activity of hydroxyl radicals and DPPH. This indicated that the antioxidant capacity of the polysaccharides was significantly higher following sulfation. Furthermore, in in vitro cell investigations, DIP exhibited no inhibitory effects on the growth of the B16 or MCF‑7 tumor cells. However, the sulfated derivatives exerted marked inhibitory effects on these cell lines. Sulfate modification may therefore contribute to an improvement in water solubility and in the antioxidant and antitumor activities of natural DIP.
本研究描述了从竹荪中提取的水不溶性多糖（DIP）制备的水溶性硫酸化多糖的特性和生物学性质。采用氯磺酸 - 吡啶法对DIP进行硫酸化。根据《中国药典》在室温下测定硫酸化衍生物的水溶性。测定了硫酸化多糖对羟基自由基和1,1 - 二苯基 - 2 - 苦基肼（DPPH）的清除活性以及还原能力。然后使用MTT法测定DIP和硫酸化衍生物对MCF - 7和B16细胞的细胞毒性和抗增殖作用。根据氯化钡 - 明胶比浊法，硫酸化多糖的取代度分别为0.584（S1 - DIP）、0.989（S2 - DIP）和1.549（S3 - DIP）。红外光谱和13C - 核磁共振表明，S - DIP的取代主要发生在C - 6位，其次是C - 4和C - 2位。与DIP相比，硫酸化衍生物的抗氧化活性显著增加。此外，S - DIPs表现出更显著的还原能力以及对羟基自由基和DPPH的清除活性。这表明多糖硫酸化后抗氧化能力显著提高。此外，在体外细胞研究中，DIP对B16或MCF - 7肿瘤细胞的生长没有抑制作用。然而，硫酸化衍生物对这些细胞系具有显著的抑制作用。因此，硫酸化修饰可能有助于提高天然DIP的水溶性、抗氧化和抗肿瘤活性。
