Veronesi Francesca, Torricelli Paola, Della Bella Elena, Pagani Stefania, Fini Milena
Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy.
Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy; Laboratory of Biocompatibility, Innovative Technologies and Advanced Therapies, Department Rizzoli RIT, Bologna, Italy.
Cytotherapy. 2015 Feb;17(2):215-23. doi: 10.1016/j.jcyt.2014.10.006. Epub 2014 Dec 4.
Tendon is a complex tissue with a reduced regenerative ability. Nowadays, little or nothing is known about the regenerative effect of adipose-derived mesenchymal stromal cells (ADSCs) on tendons.
The study aimed to evaluate the in vitro mutual interaction of ADSCs and tenocytes in standard culture conditions and a microwound healing model. Tenocyte viability, microwound recovery and the expression of genes encoding for the main extracellular matrix components and ADSC viability, differentiation and growth factor gene expression were evaluated.
The effects of ADSCs on tenocytes were observed more in the microwound healing model, in which the rate of microwound healing and the expression of decorin, tenascin and collagens were significantly increased. The influence of tenocytes on ADSCs was also found in standard culture conditions: ADSCs were directed toward a tenogenic lineage, and growth factor expression increased.
This study clarifies some aspects of the mutual interaction of ADSCs and tenocytes and provides in vitro evidence for a possible future application of ADSCs as a therapeutic strategy for tendon repair.
肌腱是一种再生能力有限的复杂组织。目前，关于脂肪来源的间充质基质细胞（ADSCs）对肌腱的再生作用知之甚少或几乎一无所知。
本研究旨在评估在标准培养条件和微创伤愈合模型中ADSCs与肌腱细胞的体外相互作用。评估了肌腱细胞活力、微创伤恢复情况以及编码主要细胞外基质成分的基因表达，以及ADSCs活力、分化和生长因子基因表达。
在微创伤愈合模型中观察到ADSCs对肌腱细胞的影响更大，其中微创伤愈合率以及核心蛋白聚糖、腱生蛋白和胶原蛋白的表达显著增加。在标准培养条件下也发现了肌腱细胞对ADSCs的影响：ADSCs被引导向肌腱生成谱系，且生长因子表达增加。
本研究阐明了ADSCs与肌腱细胞相互作用的一些方面，并为ADSCs作为肌腱修复治疗策略的未来可能应用提供了体外证据。
