在肌腱-骨界面的体外共培养模型中双向调节细胞相互作用。
Bi-directional modulation of cellular interactions in an in vitro co-culture model of tendon-to-bone interface.
机构信息
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine- Parque de Ciência e Tecnologia, Barco, Guimarães, Portugal.
ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
出版信息
Cell Prolif. 2018 Dec;51(6):e12493. doi: 10.1111/cpr.12493. Epub 2018 Aug 14.
OBJECTIVES
This work aimed at studying in vitro interactions between human tendon-derived cells (hTDCs) and pre-osteoblasts (pre-OBs) that may trigger a cascade of events involved in enthesis regeneration.
MATERIALS AND METHODS
The effect of 5 osteogenic medium (OM) conditions over the modulation of hTDCs and pre-OBs towards the tenogenic and osteogenic phenotypes, respectively, was studied. Three different medium conditions were chosen for subsequently establishing a direct co-culture system in order to study the expression of bone, tendon and interface-related markers.
RESULTS
A higher matrix mineralization and ALP activity was observed in co-cultures in the presence of OM. Higher transcription levels of bone- (ALPL, RUNX2, SPP1) and interface-related genes (ACAN, COMP) were found in co-cultures. The expression of aggrecan was influenced by the presence of OM and cell-cell interactions occurring in co-culture.
CONCLUSIONS
The present work assessed both the influence of OM on cell phenotype modulation and the importance of co-culture models while promoting cell-cell interactions and the exchange of soluble factors in triggering an interface-like phenotype to potentially modulate enthesis regeneration.
目的
本研究旨在研究人肌腱源细胞（hTDCs）和前成骨细胞（pre-OBs）之间的体外相互作用，这些相互作用可能引发参与腱骨结合部再生的一系列事件。
材料和方法
研究了 5 种成骨培养基（OM）条件对 hTDCs 和 pre-OBs 向腱形成和成骨表型的分别调节作用。选择了三种不同的培养基条件，以便随后建立直接共培养系统，以研究骨、腱和界面相关标志物的表达。
结果
在 OM 存在的情况下，共培养中观察到更高的基质矿化和碱性磷酸酶活性。共培养中发现骨（ALPL、RUNX2、SPP1）和界面相关基因（ACAN、COMP）的转录水平更高。aggrecan 的表达受到 OM 的存在和共培养中发生的细胞-细胞相互作用的影响。
结论
本研究评估了 OM 对细胞表型调节的影响，以及共培养模型的重要性，同时促进了细胞-细胞相互作用和可溶性因子的交换，以潜在地调节腱骨结合部的再生。
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