Henke W, Lang M, Dubiel W, Holzhütter H G, Gerber G
Abteilung für experimentelle Organtransplantation, Leninallee, Berlin, DDR.
Biochem Int. 1989 Apr;18(4):833-44.
In rat liver mitochondria there exists an AMP-dephosphorylating activity which converts external 5'-AMP to adenosine. It exhibits a pH optimum of 7.5 and a Km(AMP) of 0.085 mM. Furthermore, this activity is stimulated by magnesium (Km = 0.5 mM) and seems to be not affected by low concentrations of ATP or ADP. From the characteristics of the enzyme the existence of a 5'-nucleotidase in rat liver mitochondria which is localized on the outer surface of the inner mitochondrial membrane was concluded. The enzyme may be important for the production of cellular adenosine.
在大鼠肝脏线粒体中存在一种AMP去磷酸化活性，可将细胞外5'-AMP转化为腺苷。它的最适pH为7.5，Km(AMP)为0.085 mM。此外，该活性受到镁的刺激（Km = 0.5 mM），并且似乎不受低浓度ATP或ADP的影响。根据该酶的特性，得出大鼠肝脏线粒体中存在一种定位于线粒体内膜外表面的5'-核苷酸酶的结论。该酶可能对细胞腺苷的产生很重要。
