Chan Shing Fai, Huang Xiayu, McKercher Scott R, Zaidi Rameez, Okamoto Shu-Ichi, Nakanishi Nobuki, Lipton Stuart A
Neuroscience and Aging Research Center, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA.
Bioinformatics and Systems Biology Program, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA.
Genom Data. 2015 Mar 1;3:24-27. doi: 10.1016/j.gdata.2014.10.022.
[Briefly describe the contents of the Data in Brief article. Tell the reader the repository and reference number for the data in the abstract to.] The myocyte enhancer factor 2 (MEF2) family of transcription factors is highly expressed in the brain, and constitutes a key determinant of neuronal survival, differentiation, and synaptic plasticity. However, genome-wide transcriptional profiling of MEF2-regulated genes has not yet been fully elucidated, particularly at the neural stem cell stage. Here we report the results of microarray analysis comparing mRNAs isolated from human neural progenitor/stem cells (hNPCs) derived from embryonic stem cells expressing a control vector versus progenitors expressing a constitutively-active form of MEF2 (MEF2CA), which increases MEF2 activity. Microarray experiments were performed using the Illumina Human HT-12 V4.0 expression beadchip (GEO#: GSE57184). By comparing vector-control cells to MEF2CA cells, microarray analysis identified 1880 unique genes that were differentially expressed. Among these genes, 1121 genes were upregulated and 759 genes were down-regulated. Our results provide a valuable resource for identifying transcriptional targets of MEF2 in hNPCs.
[简要描述“数据简报”文章的内容。在摘要中告知读者数据的存储库和参考文献编号。] 转录因子的肌细胞增强因子2（MEF2）家族在大脑中高度表达，是神经元存活、分化和突触可塑性的关键决定因素。然而，MEF2调控基因的全基因组转录谱尚未完全阐明，特别是在神经干细胞阶段。在此，我们报告了微阵列分析的结果，该分析比较了从表达对照载体的胚胎干细胞衍生的人类神经祖细胞/干细胞（hNPC）与表达组成型活性形式的MEF2（MEF2CA）的祖细胞中分离的mRNA，MEF2CA可增加MEF2活性。使用Illumina Human HT-12 V4.0表达微珠芯片（GEO编号：GSE57184）进行微阵列实验。通过将载体对照细胞与MEF2CA细胞进行比较，微阵列分析鉴定出1880个差异表达的独特基因。在这些基因中，1121个基因上调，759个基因下调。我们的结果为鉴定hNPC中MEF2的转录靶点提供了宝贵的资源。
