Koussa Mounir A, Halvorsen Ken, Ward Andrew, Wong Wesley P
Program in Neuroscience, Department of Neurobiology, Harvard Medical School, Boston, MA, United States.
The RNA Institute, University at Albany, Albany, NY, United States.
Nat Methods. 2015 Feb;12(2):123-126. doi: 10.1038/nmeth.3209. Epub 2014 Dec 8.
We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions using a gel electrophoresis readout. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrated this low-cost, versatile, 'lab-on-a-molecule' system by characterizing ten different interactions, including a complex four-body interaction with five discernible states.
我们介绍了一种纳米级实验平台，该平台能够使用凝胶电泳读数对广泛的分子相互作用进行动力学和平衡测量。可编程的自组装DNA纳米开关既作为分子定位的模板，又作为分子缔合和解离的灵敏定量报告器。我们通过表征十种不同的相互作用，包括具有五个可分辨状态的复杂四体相互作用，展示了这种低成本、多功能的“分子上的实验室”系统。
Zotero 插件
