Angel I, Taranger M A
Department of Biology, Synthélabo Recherche (L.E.R.S.), Paris, (France).
Brain Res. 1989 Jun 26;490(2):367-72. doi: 10.1016/0006-8993(89)90256-4.
The hypothalamic response to circulating glucose and insulin levels was studied in the mouse by differentially attenuating glucose-homeostasis. The administration of glucose, 2-deoxyglucose or the alpha 2-adrenoceptor agonist UK 14.304 was accompanied by a persistent hyperglycaemia; however, an increase in insulin levels was obtained with glucose and a decrease with the other two manipulations. Both alpha 2-adrenoceptors (labeled with [3H]idazoxan) and the anorectic recognition site (labeled with [3H]mazindol) were upregulated by the three treatments. A good correlation was obtained between circulating glucose levels and either hypothalamic [3H]mazindol binding (r = 0.70, P less than 0.001) or [3H]idazoxan binding (r = 0.63, P less than 0.001), as well as between the two binding sites (r = 0.88, P less than 0.001). No correlation was obtained between circulating insulin levels and these binding sites (r = 0.18, r = 0.26, P = n.s. for [3H]mazindol and [3H]idazoxan binding, respectively). It is suggested the alpha 2-adrenoceptors and the anorectic binding sites are associated in their response to glucose as part of a hypothalamic center involved in the regulation of feeding mechanisms.
通过差异性减弱葡萄糖稳态，研究了小鼠下丘脑对循环葡萄糖和胰岛素水平的反应。给予葡萄糖、2-脱氧葡萄糖或α2-肾上腺素能受体激动剂UK 14.304后均出现持续性高血糖；然而，葡萄糖使胰岛素水平升高，而其他两种处理使其降低。三种处理均使α2-肾上腺素能受体（用[3H]咪唑克生标记）和厌食识别位点（用[3H]马吲哚标记）上调。循环葡萄糖水平与下丘脑[3H]马吲哚结合（r = 0.70，P < 0.001）或[3H]咪唑克生结合（r = 0.63，P < 0.001）之间，以及两个结合位点之间（r = 0.88，P < 0.001）均存在良好的相关性。循环胰岛素水平与这些结合位点之间无相关性（[3H]马吲哚和[3H]咪唑克生结合的r分别为0.18和0.26，P无统计学意义）。提示α2-肾上腺素能受体和厌食结合位点在对葡萄糖的反应中相关联，是参与进食机制调节的下丘脑中心的一部分。
