Lehner Małgorzata, Wisłowska-Stanek Aleksandra, Skórzewska Anna, Płaźnik Adam
Department of Neurochemistry, Institute of Psychiatry and Neurology, 9 Sobieskiego Street, 02-957 Warsaw, Poland.
Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 1b Banacha Street, 02-097 Warsaw, Poland.
Neurosci Lett. 2015 Jan 23;586:55-9. doi: 10.1016/j.neulet.2014.12.011. Epub 2014 Dec 5.
This study examined the effects of chronic restraint stress and corticosterone treatment on the apoptosis-related processes in the dentate gyrus of the rat hippocampus. This study compared high (HR) and low anxiety rats (LR) (as defined by their behaviour during the contextual fear test, i.e., the duration of a freezing response was the discriminating variable). The results demonstrate that chronic restraint stress increased the number of caspase-3 immunoreactive cells in the HR group, whereas repeated corticosterone treatment increased the number of caspase-3 immunoreactive cells in both the HR and LR groups. This finding suggests that higher susceptibility to fear stimuli predisposes rats to increased apoptosis in the hippocampus after exposure to chronic stressors. This new animal model of HR and LR rats can be used to study the mechanisms underlying the relationship between higher levels of anxiety and greater vulnerability to stress.
本研究考察了慢性束缚应激和皮质酮处理对大鼠海马齿状回中凋亡相关过程的影响。本研究比较了高焦虑大鼠（HR）和低焦虑大鼠（LR）（根据它们在情境恐惧测试中的行为定义，即僵住反应的持续时间为区分变量）。结果表明，慢性束缚应激增加了HR组中半胱天冬酶-3免疫反应性细胞的数量，而重复给予皮质酮处理则增加了HR组和LR组中半胱天冬酶-3免疫反应性细胞的数量。这一发现表明，对恐惧刺激的更高易感性使大鼠在暴露于慢性应激源后海马体中的细胞凋亡增加。这种新的HR和LR大鼠动物模型可用于研究高焦虑水平与更大应激易感性之间关系的潜在机制。
