Strack A M, Sawyer W B, Platt K B, Loewy A D
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Brain Res. 1989 Jul 10;491(2):274-96. doi: 10.1016/0006-8993(89)90063-2.
The CNS cell groups that innervate the sympathoadrenal preganglionic neurons of rats were identified by a transneuronal viral cell body labeling technique combined with neurotransmitter immunohistochemistry. Pseudorabies virus was injected into the adrenal gland. This resulted in retrograde viral infections of the ipsilateral sympathetic preganglionic neurons (T4-T13) and caused retrograde transneuronal cell body infections in 5 areas of the brain: the caudal raphe nuclei, ventromedial medulla, rostral ventrolateral medulla, A5 cell group, and paraventricular hypothalamic nucleus (PVH). In the spinal cord, the segmental distribution of virally infected neurons was the same as the retrograde cell body labeling observed following Fluoro-gold injections in the adrenal gland except there was almost a 300% increase in the number of cells labeled and a shift in cell group distribution. These results imply there are local interneurons that regulate the sympathoadrenal preganglionic neurons. In the medulla oblongata, serotonin (5-HT)-, substance P (SP)-, thyrotropin-releasing hormone-, Met-enkephalin-, and somatostatin-immunoreactive neurons of the raphe pallidus and raphe obscurus nuclei and the ventromedial medulla were infected. In the ventromedial and rostral ventrolateral medulla, immunoreactive phenylethanolamine-N-methyltransferase, SP, neuropeptide Y, somatostatin, and enkephalin neurons were infected. The A5 noradrenergic cells were labeled, as were some somatostatin-immunoreactive neurons in this area. In the were infected. The A5 noradrenergic cells were labeled, as were some somatostatin-immunoreactive neurons in this area. In the hypothalamus, tyrosine hydroxylase- and SP-immunoreactive neurons of the dorsal parvocellular PVH were infected. Only a few immunoreactive vasopressin, oxytocin, Met-enkephalin, neurotensin, and somatostatin PVH neurons were labeled.
通过跨神经元病毒细胞体标记技术结合神经递质免疫组织化学，确定了支配大鼠交感肾上腺节前神经元的中枢神经系统细胞群。将伪狂犬病病毒注入肾上腺。这导致同侧交感节前神经元（T4 - T13）发生逆行病毒感染，并在脑的5个区域引起逆行跨神经元细胞体感染：尾侧中缝核、延髓腹内侧、延髓头端腹外侧、A5细胞群和下丘脑室旁核（PVH）。在脊髓中，病毒感染神经元的节段分布与在肾上腺注射荧光金后观察到的逆行细胞体标记相同，只是标记细胞数量增加了近300%，且细胞群分布发生了变化。这些结果表明存在调节交感肾上腺节前神经元的局部中间神经元。在延髓，中缝苍白核、中缝隐核和延髓腹内侧的5-羟色胺（5-HT）、P物质（SP）、促甲状腺激素释放激素、甲硫氨酸脑啡肽和生长抑素免疫反应性神经元被感染。在延髓腹内侧和头端腹外侧，苯乙醇胺-N-甲基转移酶、SP、神经肽Y、生长抑素和脑啡肽免疫反应性神经元被感染。A5去甲肾上腺素能细胞被标记，该区域的一些生长抑素免疫反应性神经元也被标记。在下丘脑，背侧小细胞PVH的酪氨酸羟化酶和SP免疫反应性神经元被感染。只有少数血管加压素、催产素、甲硫氨酸脑啡肽、神经降压素和生长抑素PVH免疫反应性神经元被标记。
