Strack A M, Sawyer W B, Hughes J H, Platt K B, Loewy A D
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Brain Res. 1989 Jul 3;491(1):156-62. doi: 10.1016/0006-8993(89)90098-x.
Pseudorabies virus (PRV) injections of various sympathetic ganglia and the adrenal gland were made in rats. These produced immunohistochemically detectable retrograde viral infections of ipsilateral sympathetic preganglionic neurons (SPNs) and transneuronal infections of the specific sets of second order neurons in the spinal cord and brain that innervate the infected SPNs. Five cell groups in the brain appear to regulate the entire sympathetic outflow: the paraventricular hypothalamic nucleus (PVH), A5 noradrenergic cell group, caudal raphe region, rostral ventrolateral medulla, and ventromedial medulla. In addition, local interneurons in laminae VII and X of the spinal cord are also involved. Other CNS areas also became transneuronally labeled after infections of certain sympathetic ganglia, most notably the superior cervical and stellate ganglia. These areas include the central gray matter and lateral hypothalamic area. The zona incerta was uniquely labeled after stellate ganglion infections. The cell body labeling was specific. This specificity was demonstrated in the PVH where the neurons of the parvocellular PVH that form the descending sympathetic pathway were labeled in a topographic fashion. Finally, we demonstrate that the retrograde transneuronal viral cell body labeling method can be used simultaneously with either neuropeptide transmitter or transmitter synthetic enzyme immunohistochemistry.
在大鼠身上对各种交感神经节和肾上腺进行伪狂犬病病毒（PRV）注射。这些注射导致同侧交感神经节前神经元（SPN）出现免疫组织化学可检测到的逆行病毒感染，以及脊髓和大脑中支配受感染SPN的特定二级神经元组出现跨神经元感染。大脑中的五个细胞群似乎调节着整个交感神经输出：室旁下丘脑核（PVH）、A5去甲肾上腺素能细胞群、尾侧中缝区域、延髓头端腹外侧区和延髓腹内侧区。此外，脊髓第VII和X层的局部中间神经元也参与其中。在某些交感神经节（最显著的是颈上神经节和星状神经节）感染后，其他中枢神经系统区域也出现了跨神经元标记。这些区域包括中央灰质和下丘脑外侧区。星状神经节感染后，未定带被独特地标记。细胞体标记具有特异性。这种特异性在PVH中得到了证明，其中形成下行交感神经通路的小细胞PVH神经元以拓扑方式被标记。最后，我们证明逆行跨神经元病毒细胞体标记方法可以与神经肽递质或递质合成酶免疫组织化学同时使用。
