Crooke S T, Bennett C F
Department of Molecular Pharmacology, Smith Kline & French Laboratories, Pennsylvania.
Cell Calcium. 1989 Jul;10(5):309-23. doi: 10.1016/0143-4160(89)90057-2.
Procaryotic and eucaryotic cells have evolved multiple pathways for communication with their external environment. The inositol 1,4,5-trisphosphate/diacylglycerol second messenger system is an example of such a signal transduction pathway which is present in multicellular eucaryotic organisms. Binding of an agonist to a specific cell surface receptor promotes rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate. The pivotal enzyme for this second messenger system is phosphoinositide-specific phospholipase C which hydrolyzes phosphatidylinositol 4,5-bisphosphate to generate the two second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Recently, much progress has been made in the purification, characterization and cDNA cloning of multiple PI-PLC isoenzymes. The results of the recent studies on phosphoinositide-specific phospholipase C are reviewed.
原核细胞和真核细胞已经进化出多种与外部环境进行通讯的途径。肌醇1,4,5-三磷酸/二酰基甘油第二信使系统就是这样一种存在于多细胞真核生物中的信号转导途径的例子。激动剂与特定细胞表面受体的结合促进磷脂酰肌醇4,5-二磷酸的快速水解。这个第二信使系统的关键酶是磷脂酰肌醇特异性磷脂酶C，它将磷脂酰肌醇4,5-二磷酸水解生成两种第二信使，即肌醇1,4,5-三磷酸和二酰基甘油。最近，在多种PI-PLC同工酶的纯化、特性鉴定和cDNA克隆方面取得了很大进展。本文综述了近期关于磷脂酰肌醇特异性磷脂酶C的研究结果。
