Frontera Jimena Laura, Cervino Ailen Soledad, Jungblut Lucas David, Paz Dante Agustín
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina.
Laboratorio de Biología del Desarrollo, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Ann Anat. 2015 Mar;198:41-8. doi: 10.1016/j.aanat.2014.10.010. Epub 2014 Nov 13.
Olfactory epithelium has the capability to continuously regenerate olfactory receptor neurons throughout life. Adult neurogenesis results from proliferation and differentiation of neural stem cells, and consequently, olfactory neuroepithelium offers an excellent opportunity to study neural regeneration and the factors involved in the maintenance and regeneration of all their cell types. We analyzed the expression of BDNF in the olfactory system under normal physiological conditions as well as during a massive regeneration induced by chemical destruction of the olfactory epithelium in Xenopus laevis larvae. We described the expression and presence of BDNF in the olfactory epithelium and bulb. In normal physiological conditions, sustentacular (glial) cells and a few scattered basal (stem) cells express BDNF in the olfactory epithelium as well as the granular cells in the olfactory bulb. Moreover, during massive regeneration, we demonstrated a drastic increase in basal cells expressing BDNF as well as an increase in BDNF in the olfactory bulb and nerve. Together these results suggest an important role of BDNF in the maintenance and regeneration of the olfactory system.
嗅觉上皮具有在一生中持续再生嗅觉受体神经元的能力。成体神经发生源于神经干细胞的增殖和分化，因此，嗅觉神经上皮为研究神经再生以及维持和再生其所有细胞类型所涉及的因素提供了绝佳机会。我们分析了非洲爪蟾幼体在正常生理条件下以及嗅觉上皮化学损伤诱导的大规模再生过程中嗅觉系统中脑源性神经营养因子（BDNF）的表达情况。我们描述了BDNF在嗅觉上皮和嗅球中的表达及存在情况。在正常生理条件下，支持（神经胶质）细胞和一些散在的基底（干）细胞在嗅觉上皮中表达BDNF，嗅球中的颗粒细胞也表达BDNF。此外，在大规模再生过程中，我们证明了表达BDNF的基底细胞急剧增加，同时嗅球和神经中的BDNF也增加。这些结果共同表明BDNF在嗅觉系统的维持和再生中起重要作用。
