Department of Otolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Neurosci Lett. 2012 May 10;516(1):45-9. doi: 10.1016/j.neulet.2012.03.051. Epub 2012 Mar 28.
To further understand the roles of growth factors in the olfactory neurogenesis, we studied the mRNA levels of diverse genes in olfactory bulb (OB) and olfactory epithelium (OE) during the regeneration process of OE. mRNA expression levels of various genes in the OB and OE during the regeneration processes of OE from damage induced by methimazole administration were studied by DNA microarray analysis. The results were confirmed by quantitative real-time RT-PCR and immunohistochemistry. Expression levels of various genes dramatically changed during the observation period. Among them, mRNA expression of BDNF dramatically increased in OE during the first 7 days and then decreased. In contrast, mRNA expression of BDNF in OB significantly decreased during the first 7 days after administration and then gradually increased. The changes in the mRNA levels of OMP in OB precisely followed those of OMP in OE and OB. The present results suggest that BDNF in OE contributes to the early stage of regeneration, and BDNF in OB has its role in the late stage of regeneration of olfactory receptor neurons (ORNs).
为了进一步了解生长因子在嗅神经发生中的作用，我们研究了嗅球（OB）和嗅上皮（OE）在OE 再生过程中的多种基因的 mRNA 水平。通过 DNA 微阵列分析研究了由甲巯咪唑给药引起的 OE 损伤后的 OB 和 OE 中各种基因在 OE 再生过程中的 mRNA 表达水平。结果通过定量实时 RT-PCR 和免疫组织化学得到了证实。在观察期间，各种基因的表达水平发生了显著变化。其中，BDNF 的 mRNA 表达在 OE 中在第 1 天至第 7 天期间急剧增加，然后减少。相比之下，BDNF 的 mRNA 表达在 OB 中在给药后第 1 天至第 7 天期间显著减少，然后逐渐增加。OB 中 OMP 的 mRNA 水平的变化与 OE 和 OB 中 OMP 的变化完全一致。这些结果表明，OE 中的 BDNF 有助于再生的早期阶段，而 OB 中的 BDNF 在嗅感觉神经元（ORNs）的再生晚期发挥作用。
