Liu Xiaoyan, Lv Jinshun, Xu Jiaxing, Zhang Tong, Deng Yuanfang, He Jianlong
Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China,
Appl Biochem Biotechnol. 2015 Mar;175(5):2347-56. doi: 10.1007/s12010-014-1430-0. Epub 2014 Dec 7.
In this study, citric acid was produced from waste cooking oil by Yarrowia lipolytica SWJ-1b. To get the maximal yield of citric acid, the compositions of the medium for citric acid production were optimized, and our results showed that extra nitrogen and magnesium rather than vitamin B1 and phosphate were needed for CA accumulation when using waste cooking oil. The results also indicated that the optimal initial concentration of the waste cooking oil in the medium for citric acid production was 80.0 g/l, and the ideal inoculation size was 1 × 10(7) cells/l of medium. We also reported that during 10-l fermentation, 31.7 g/l of citric acid, 6.5 g/l of isocitric acid, 5.9 g/l of biomass, and 42.1 g/100.0 g cell dry weight of lipid were attained from 80.0 g/l of waste cooking oil within 336 h. At the end of the fermentation, 94.6 % of the waste cooking oil was utilized by the cells of Y. lipolytica SWJ-1b, and the yield of citric acid was 0.4 g/g waste cooking oil, which suggested that waste cooking oil was a suitable carbon resource for citric acid production.
在本研究中，解脂耶氏酵母SWJ-1b利用废弃食用油生产柠檬酸。为了获得柠檬酸的最大产量，对柠檬酸生产培养基的成分进行了优化，我们的结果表明，使用废弃食用油时，积累柠檬酸需要额外的氮和镁，而不是维生素B1和磷酸盐。结果还表明，柠檬酸生产培养基中废弃食用油的最佳初始浓度为80.0 g/l，理想接种量为1×10(7)个细胞/l培养基。我们还报告称，在10升发酵过程中，在336小时内从80.0 g/l废弃食用油中获得了31.7 g/l柠檬酸、6.5 g/l异柠檬酸、5.9 g/l生物量以及42.1 g/100.0 g细胞干重的脂质。在发酵结束时，解脂耶氏酵母SWJ-1b细胞利用了94.6%的废弃食用油，柠檬酸产量为0.4 g/g废弃食用油，这表明废弃食用油是生产柠檬酸的合适碳源。
