Taverna Domenico, Pollins Alonda C, Sindona Giovanni, Caprioli Richard M, Nanney Lillian B
Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria , Via P. Bucci, cubo 12/D, Arcavacata di Rende, CS, 87036, Italy.
J Proteome Res. 2015 Feb 6;14(2):986-96. doi: 10.1021/pr5010218. Epub 2014 Dec 19.
Imaging mass spectrometry (IMS) was employed for the analysis of frozen skin biopsies to investigate the differences between stage IV pressure ulcers that remain stalled, stagnant, and unhealed versus those exhibiting clinical and histological signs of improvement. Our data reveal a rich diversity of proteins that are dynamically modulated, and we selectively highlight a family of calcium binding proteins (S-100 molecules) including calcyclin (S100-A6), calgranulins A (S100-A8) and B (S100-A9), and calgizzarin (S100-A11). IMS allowed us to target three discrete regions of interest: the wound bed, adjacent dermis, and hypertrophic epidermis. Plots derived using unsupervised principal component analysis of the global protein signatures within these three spatial niches indicate that these data from wound signatures have potential as a prognostic tool since they appear to delineate wounds that are favorably responding to therapeutic interventions versus those that remain stagnant or intractable in their healing status. Our discovery-based approach with IMS augments current knowledge of the molecular signatures within pressure ulcers while providing a rationale for a focused examination of the role of calcium modulators within the context of impaired wound healing.
成像质谱（IMS）用于分析冷冻皮肤活检样本，以研究处于停滞、无进展且未愈合状态的IV期压疮与那些呈现临床和组织学改善迹象的IV期压疮之间的差异。我们的数据揭示了大量动态调节的蛋白质，我们特别强调了一类钙结合蛋白（S-100分子），包括钙环蛋白（S100-A6）、钙粒蛋白A（S100-A8）和B（S100-A9）以及钙锌蛋白（S100-A11）。IMS使我们能够针对三个不同的感兴趣区域：伤口床、相邻真皮和肥厚性表皮。利用这三个空间生态位内的全局蛋白质特征进行无监督主成分分析得出的图表明，这些来自伤口特征的数据有潜力作为一种预后工具，因为它们似乎能够区分对治疗干预有良好反应的伤口与那些愈合状态停滞或难治的伤口。我们基于发现的IMS方法增加了目前对压疮内分子特征的认识，同时为在伤口愈合受损的背景下重点研究钙调节剂的作用提供了理论依据。
