Kumar Sumit, Kumar Akhilesh, Kumar Naveen, Singh Praveen, Singh Thakur Uttam, Singh Bhoj Raj, Gupta Piyush Kumar, Thakur Vijay Kumar
Division of Medicine, ICAR-Indian Veterinary Research Institute Izatnagar, 243122, Bareilly, Uttar Pradesh, India.
Division of Surgery, ICAR-Indian Veterinary Research Institute Izatnagar, 243122, Bareilly, Uttar Pradesh, India.
Vet Res Commun. 2022 Dec;46(4):1033-1049. doi: 10.1007/s11259-022-09952-1. Epub 2022 Jul 7.
Since ancient times, medicinal plants are widely accepted to promote the health and wellness of animals and mankind. The medicinal plant-based therapies have limitations of delayed onset of action, inconsistent absorption, low bioavailability, oxidation, and poor solubility. The encapsulation studies suggested improved efficacy. Therefore, the present study attempts to evaluate the efficacy of Curcuma longa extracts encapsulated in Ethosome on wound healing model compared to crude extract. The Curcuma longa extract swere prepared by cold percolation method and total curcuminoid content was determined by Reverse phase-HPLC. Three Ethosomal suspensions (ETS1, ETS2, and ETS3) were prepared and characterized for particle distribution, morphology, and absorption spectrum by Zetasizer, Scanning Electron Microscopy, and FTIR respectively. The Ethosomal suspension with the highest entrapment efficiency was applied topically at a varying concentrations (0.25, 0.5, and 1 g/cm) on the surgically created wounds in rats. The efficacy of wound healing was evaluated by clinical observation, macroscopic evaluation of granulation tissue, colour digital image processing, and histology. The methanolic extract of Curcuma longa showed better antibacterial potential than ethanolic and aqueous. The total Curcuminoid content in the Curcuma longa rhizome was 4.03%. The size, PDI, zeta potential, and viscosity of Ethosomal suspension ranged from 34.8 to 371 nm, 0.236-1.178, 15.6-36.8mV, and 0.8460-0.8510, respectively. The ETS3 was found the most optimum combination with the highest entrapment efficiency and the topical application at a dose rate of 0.5 g/cm and 1.0 g/cm resulted in comparable wound contracture, pain score, histopathological score as compared to control groups.It was concluded that the Curcuma longa encapsulation in Ethosome resulted in improved wound appearance, granulation tissue score, and appearance with a shortened period of wound resolution at the cellular level as compared to crude extract.
自古以来，药用植物被广泛认为可促进动物和人类的健康。基于药用植物的疗法存在起效延迟、吸收不一致、生物利用度低、氧化和溶解性差等局限性。包封研究表明其疗效有所提高。因此，本研究试图评估与粗提物相比，脂质体包裹的姜黄提取物在伤口愈合模型上的疗效。姜黄提取物采用冷渗漉法制备，总姜黄素含量通过反相高效液相色谱法测定。制备了三种脂质体混悬液（ETS1、ETS2和ETS3），并分别通过zetasizer、扫描电子显微镜和傅里叶变换红外光谱对其粒径分布、形态和吸收光谱进行了表征。将包封率最高的脂质体混悬液以不同浓度（0.25、0.5和1 g/cm）局部应用于大鼠手术造成的伤口。通过临床观察、肉芽组织的宏观评估、彩色数字图像处理和组织学来评估伤口愈合的疗效。姜黄的甲醇提取物显示出比乙醇提取物和水提取物更好的抗菌潜力。姜黄根茎中的总姜黄素含量为4.03%。脂质体混悬液的粒径、多分散指数、zeta电位和粘度分别为34.8至371 nm、0.236 - 1.178、15.6 - 36.8 mV和0.8460 - 0.8510。发现ETS3是包封率最高的最适宜组合，与对照组相比，以0.5 g/cm和1.0 g/cm的剂量率局部应用导致了相当的伤口收缩、疼痛评分和组织病理学评分。得出的结论是，与粗提物相比，脂质体包裹姜黄可改善伤口外观、肉芽组织评分，并在细胞水平上缩短伤口愈合时间。
